Department of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Department of Immunology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia.
Trop Biomed. 2021 Mar 1;38(1):42-49. doi: 10.47665/tb.38.1.008.
Chikungunya is an infection caused by chikungunya virus (CHIKV). Although chikungunya has affected many countries in recent times, specific treatment or licensed vaccine are nonexistent. In this study the potential antiviral properties of Tualang honey against in vitro CHIKV infection was evaluated. Cytotoxic test was performed using the XTT Cell Viability assay to determine maximum non-toxic dose (MNTD) in Vero cells. Using plaque assay, the potential antiviral activities of Tualang honey at various non-toxic concentrations and treatment regimens were evaluated. Tualang honey demonstrated virucidal effect with maximum inhibition CHIKV observed was 99.71% (p< 0.05). Tualang honey also had a prophylactic property by conferring protection to Vero cells during pre-treatment assay, resulting in up to 98.22% reduction of CHIKV replication under certain treatment regimen. Furthermore, Tualang honey exhibited anti-viral activities, with as much as 94.87% inhibition following post-treatment assay of Tualang honey in CHIKV-infected Vero cells. Additionally, Tualang honey also affected viral entry up to 82.21% after 48 hours of infection. These results suggest that Tualang honey has wide anti-CHIKV activities in Vero cells and exerts its effect through different mechanisms although these need to be further validated in other cells or model of CHIKV infection.
基孔肯雅热是由基孔肯雅病毒（CHIKV）引起的感染。尽管基孔肯雅热在最近一段时间已经影响了许多国家，但仍没有特定的治疗方法或许可疫苗。在这项研究中，评估了塔朗蜂蜜对体外 CHIKV 感染的潜在抗病毒特性。使用 XTT 细胞活力测定法进行细胞毒性试验，以确定 Vero 细胞中的最大无毒剂量（MNTD）。使用蚀斑试验，评估了塔朗蜂蜜在各种无毒浓度和治疗方案下的潜在抗病毒活性。塔朗蜂蜜表现出病毒杀灭作用，观察到的最大 CHIKV 抑制率为 99.71%（p<0.05）。塔朗蜂蜜还具有预防特性，通过在预处理试验中为 Vero 细胞提供保护，在某些治疗方案下，可将 CHIKV 复制减少高达 98.22%。此外，塔朗蜂蜜在 CHIKV 感染的 Vero 细胞中进行治疗后试验时，表现出高达 94.87%的抗病毒活性。此外，塔朗蜂蜜在感染后 48 小时还能影响病毒进入，抑制率高达 82.21%。这些结果表明，塔朗蜂蜜在 Vero 细胞中具有广泛的抗 CHIKV 活性，并且通过不同的机制发挥作用，尽管这些机制需要在其他细胞或 CHIKV 感染模型中进一步验证。
