Republic of Turkey, Ministry of Agriculture and Forestry, Institute of Foot and Mouth Disease (SAP), Ankara, Turkey.
Harran University, Faculty of Veterinary Medicine, Virology Department, S anl ¸ urfa, Turkey.
Trop Biomed. 2021 Mar 1;38(1):154-159. doi: 10.47665/tb.38.1.027.
Despite the widespread use of the conventional inactivated foot-and-mouth disease (FMD) vaccine, its immunogenicity is poor and the duration of its protection is short. In this study, humoral response to commercial ready-to-use Montanide ISA 201 VG and Montanide ISA 61 VG oil adjuvants and a common adjuvant Montanide ISA 206 VG developed by Seppic Inc., were evaluated for FMD antigens in sheep and double oil emulsion (w/o/w) formulations of Montanide ISA 201 and 206 and single oil emulsion (w/o) of Montanide ISA 61 have been prepared by using current FMDV antigens (O/TUR/07, A/ASIA/G-VII, A/TUR/16 and ASIA/ TUR/15). The animals (n=48) were vaccinated subcutaneously with formulations and five sheep were maintained as an unvaccinated control group. Blood samples were taken at day 0, 7, 14, 21, 28, 60, 90, 120 and 150. Virus neutralization and liquid phase blocking ELISA tests were used to compare antibody response to vaccines prepared by using different Montanide mineral oils. The results showed that vaccines prepared by using Montanide ISA 61 and 201 gave better antibody response to FMD antigens than Montanide ISA 206 formulation, although results were not statistically significant for certain days of sampling. Moreover, the overall type O antibody response of Montanide ISA 201 was found to be superior to Montanide ISA 61.
尽管常规的口蹄疫（FMD）灭活疫苗得到了广泛应用，但它的免疫原性较差，保护期较短。本研究评估了用于绵羊的商品化即用型 Montanide ISA 201 VG 和 Montanide ISA 61 VG 油佐剂以及由 Seppic Inc. 开发的普通佐剂 Montanide ISA 206 VG 的体液免疫反应，制备了 Montanide ISA 201 和 206 的双油乳剂（w/o/w）和 Montanide ISA 61 的单油乳剂（w/o），使用当前的 FMDV 抗原（O/TUR/07、A/ASIA/G-VII、A/TUR/16 和 ASIA/TUR/15）。将动物（n=48）皮下接种制剂，5 只羊作为未接种对照组。在第 0、7、14、21、28、60、90、120 和 150 天采集血液样本。使用病毒中和试验和液相阻断 ELISA 试验比较了使用不同 Montanide 矿物油制备的疫苗的抗体反应。结果表明，使用 Montanide ISA 61 和 201 制备的疫苗对 FMD 抗原的抗体反应优于 Montanide ISA 206 制剂，尽管在某些采样日的结果没有统计学意义。此外，发现 Montanide ISA 201 的 O 型总抗体反应优于 Montanide ISA 61。
