Medical Laboratory Sciences Department, College of Health Sciences, University of Sharjah, PO Box 27272, Sharjah, United Arab Emirates.
Trop Biomed. 2021 Mar 1;38(1):180-182. doi: 10.47665/tb.38.1.030.
The aim of this study was to detect and characterize Giardia lamblia in animals in the UAE. Eighty-seven fecal samples were tested for G. lamblia using the conserved fragment of small subunit (SSU)-rRNA by nested PCR. Giardia-positive isolates were genotyped for assemblages A and B using assemblage specific primers of the triosephosphate isomerase (tpi) gene. Thirty samples (34.5%) were positive for G. lamblia. Conversely, neither genotype A nor B were detected using tpi genotyping on the studied samples. Further investigations are required using higher number of samples including both human and animals in the country taking into consideration the analysis of other genotypes to provide more detailed understanding about the zoonotic transmission of this parasite.
本研究旨在检测和鉴定阿联酋动物体内的蓝氏贾第鞭毛虫。采用巢式 PCR 法，用小亚基（SSU）-rRNA 保守片段对 87 份粪便样本进行蓝氏贾第鞭毛虫检测。利用三磷酸甘油醛异构酶（tpi）基因的种特异性引物，对贾第虫阳性分离株进行 A 型和 B 型的基因分型。30 份样本（34.5%）为蓝氏贾第鞭毛虫阳性。相反，在研究样本中使用 tpi 基因分型既未检测到 A 型也未检测到 B 型。需要使用更多的样本，包括该国的人和动物，进行进一步的调查，同时考虑分析其他基因型，以更详细地了解这种寄生虫的人畜共患传播。
