Transcript profiles analysis of citrus aquaporins in response to fruit water loss during storage.

Water loss is an essential factor that affects the maintenance of quality of citrus fruit during postharvest handling and storage. Aquaporins (AQPs) play an important role in the transport of water across membranes. However, the expression profiling of AQPs is incomplete for citrus fruits during storage. In this study, a post-harvest storage experiment was performed using sweet orange fruits to determine changes in water loss and fruit quality. Also, genome-wide expression analysis of CsAQP genes was carried out in fruit of different citrus varieties during storage. Low humidity storage conditions accelerated the postharvest water loss and texture decline and increased the TSS content in the fruit. A total of 39 non-redundant CsAQP genes were identified. A comprehensive analysis of these genes demonstrated that all AQPs had conserved filter motifs in the different citrus varieties examined. Moreover, multiple expression analysis revealed AQPs had complex expression profiles upon water loss in citrus fruit, being time-specific in tight-skin varieties (orange and pomelo varieties), tissue-specific between peel and pulp, and variety-specific between loose-skin (mandarin varieties) and tight-skin varieties (such as sweet orange and pummelo). These results indicated that the relative humidity in storage environment affected the postharvest water loss and quality of citrus fruit. Besides, the alternation in AQPs expression may partially account for the different water loss ratio in citrus varieties and the transfer of water between the peel and the pulp of citrus fruit during storage.