All-Russian Institute of Plant Protection, St. Petersburg-Pushkin 196608, Russia.
Institute for Cereal Crops Improvement, School of Plant Sciences and Food Security, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Plant Dis. 2021 May;105(5):1495-1504. doi: 10.1094/PDIS-09-20-1927-RE. Epub 2021 Apr 2.
Variability of the Russian population of from durum wheat was studied with virulence and simple sequence repeat (SSR) markers. The pathogen was sampled during 2017 to 2019 in all regions with sizable durum wheat () growing areas from winter (North Caucasus) and spring (Middle Volga, Ural, and West Siberia) wheat. A total of 474 isolates were tested on a set of 20 -gene lines. Molecular genotypes for 105 selected isolates were determined at 11 SSR loci. Variable virulence/avirulence reaction was observed only on three -gene lines, whereas just five SSR loci were polymorphic with two alleles at each. Seven different virulence phenotypes and 11 SSR genotypes were found among 474 and 105 isolates, respectively, indicating a very low variability of the pathogen. One virulence phenotype and three SSR genotypes occurred in all Russian regions. However, two phenotypes were specific to the European regions of Russia (North Caucasus and Middle Volga), while another two were found only in the Asian part of Russia (Ural and West Siberia). Significant differentiation between six populations of . from durum wheat in the Asian and European (mainly North Caucasus) regions was also shown with numerous metrics and approaches for data with and without clone correction. Relationships among the regional populations of . from durum wheat established with virulence phenotypes significantly associated with those for SSR genotypes and was similar to the relationships among the regional populations of the pathogen from common wheat.
从硬粒小麦中分离出的 种群变异性研究，利用了致病力和简单重复序列（SSR）标记。2017 年至 2019 年，在所有冬小麦（北高加索地区）和春小麦（中伏尔加地区、乌拉尔地区和西西伯利亚地区）种植面积较大的地区，对病原菌进行了采样。总共测试了 474 个分离物对一组 20 个基因系。在 11 个 SSR 位点上确定了 105 个选定分离物的分子基因型。只有在三个基因系上观察到可变的毒力/非毒性反应，而只有五个 SSR 位点具有每个位点两个等位基因的多态性。在 474 个和 105 个分离物中分别发现了 7 种不同的致病表型和 11 种 SSR 基因型，表明病原菌的变异性非常低。一种致病表型和三种 SSR 基因型存在于所有俄罗斯地区。然而，两种表型仅存在于俄罗斯的亚洲地区（乌拉尔和西西伯利亚），而另外两种表型仅存在于俄罗斯的欧洲地区（北高加索和中伏尔加地区）。来自硬粒小麦的. 种群在亚洲和欧洲（主要是北高加索地区）的六个种群也表现出显著的分化，使用了多种方法和指标来处理有和无克隆校正的数据。通过与 SSR 基因型显著相关的致病表型建立的硬粒小麦来自. 种群的区域种群关系，与来自普通小麦的病原菌的区域种群关系相似。
