Phytopathology. 2007 Sep;97(9):1141-9. doi: 10.1094/PHYTO-97-9-1141.
ABSTRACT Isolates of Puccinia triticina collected from common wheat in the Central Asia countries of Kazakhstan, Uzbekistan, Tajikistan, and Kyrgyzstan and the Caucasus countries of Azerbaijan, Georgia, and Armenia were tested for virulence to 20 isolines of Thatcher wheat with different leaf rust resistance genes and molecular genotype at 23 simple sequence repeat (SSR) loci. After clone correction within each country, 99 isolates were analyzed for measures of population diversity, variation at single SSR loci, and for genetic differentiation of virulence phenotypes and SSR genotypes. Isolates from Central Asia and the Caucasus were also compared with 16 P. triticina isolates collected from common wheat in North America that were representative of the virulence and molecular variation in this region and two isolates collected from durum wheat in France and the United States. Populations from the Caucasus, Uzbekistan, Tajikistan, and Kyrgyzstan were not significantly (P > 0.05) differentiated for SSR variation with F(st) and R(st) statistics. Populations from the Caucasus, Uzbekistan, Tajikistan, and Kyrgyzstan were significantly (P < 0.05) differentiated from the populations in South and North Kazakhstan for SSR variation. All populations from Central Asia and the Caucasus were significantly differentiated from the North American isolates and isolates from durum wheat for SSR variation and virulence phenotypes. There was a correlation between virulence phenotype and SSR genotype among individual isolates and at the population level. Mountain barriers may account for the differentiation of P. triticina geographic populations in Central Asia and the Caucasus.
摘要 从哈萨克斯坦、乌兹别克斯坦、塔吉克斯坦和吉尔吉斯斯坦等中亚国家以及阿塞拜疆、格鲁吉亚和亚美尼亚等高加索国家的普通小麦中采集的节节麦柄锈菌分离物，对具有不同叶锈病抗性基因和 23 个简单重复序列（SSR）位点分子基因型的 20 个 Thatcher 小麦品系进行了毒性测试。在每个国家进行克隆校正后，分析了 99 个分离物的种群多样性、单个 SSR 位点的变异以及毒性表型和 SSR 基因型的遗传分化。来自中亚和高加索的分离物还与来自北美普通小麦的 16 个节节麦柄锈菌分离物进行了比较，这些分离物代表了该地区的毒性和分子变异，以及从法国和美国采集的两个硬粒小麦分离物。来自高加索、乌兹别克斯坦、塔吉克斯坦和吉尔吉斯斯坦的 SSR 变异种群的 F（st）和 R（st）统计数据没有显著差异（P>0.05）。来自高加索、乌兹别克斯坦、塔吉克斯坦和吉尔吉斯斯坦的 SSR 变异种群与南哈萨克斯坦和北哈萨克斯坦的种群有显著差异（P<0.05）。来自中亚和高加索的所有种群在 SSR 变异和毒性表型方面均与北美分离物和硬粒小麦分离物显著不同。在个体分离物和种群水平上，毒性表型和 SSR 基因型之间存在相关性。山脉屏障可能是导致中亚和高加索地区节节麦柄锈菌地理种群分化的原因。
