Maeda R, Hayashi Y, Shibuya T
Department of Parasitology, Teikyo University School of Medicine, Tokyo, Japan.
Jpn J Exp Med. 1988 Feb;58(1):39-44.
The longevity and periodicity of microfilaremia were examined in the jird infected with Brugia malayi to be used for assessing the filaricides. Jirds 4 to 6 weeks old were inoculated subcutaneously with 100 to 200 infective larvae of B. malayi. Microfilariae were present in 75 out of 94 jirds observed over 3 years and high microfilaremia, with 10 mf/microliters or higher, developed in 43 out of 75 jirds. Such a high level of microfilaremia was necessary for narrowing the variation of microfilaria counts among the blood samples. The microfilaria negative jirds, 4 months after inoculation, were abandoned, because in those cases where they became patent later the microfilaria density did not reach an appropriate level. The selected jirds were used for experiment from 6 to 15 months after inoculation when most of them revealed the maximal count of microfilariae. The jirds that failed to develop microfilaremia to the level of 10 mf/microliters by 9 months after inoculation were also abandoned because they did not continue the appropriate level of microfilaremia even when they reached this level later. Although a significant periodicity was observed only in of 10 jirds examined by the Aikat and Das method, the peak hour of microfilaria density was observed in most animals in the afternoon and the time was nearly the same in each animal. Therefore, the blood sampling would be performed preferably in the afternoon.
为了评估杀丝虫剂，研究了感染马来布鲁线虫的沙鼠体内微丝蚴血症的持续时间和周期性。对4至6周龄的沙鼠皮下接种100至200条马来布鲁线虫感染性幼虫。在3年观察期内，94只沙鼠中有75只出现微丝蚴，75只沙鼠中有43只出现高微丝蚴血症（微丝蚴计数≥10条/微升）。如此高的微丝蚴血症水平对于缩小血样中微丝蚴计数的变异是必要的。接种后4个月微丝蚴阴性的沙鼠被淘汰，因为在这些沙鼠后来出现微丝蚴血症时，其微丝蚴密度未达到合适水平。接种后6至15个月，当大多数沙鼠微丝蚴计数达到最高时，选择这些沙鼠用于实验。接种后9个月微丝蚴血症未达到10条/微升水平的沙鼠也被淘汰，因为即使它们后来达到该水平，也未持续保持合适的微丝蚴血症水平。尽管采用艾卡特和达斯方法检查的10只沙鼠中仅1只观察到明显的周期性，但大多数动物微丝蚴密度的高峰时间出现在下午，且每只动物的时间几乎相同。因此，血样采集最好在下午进行。
