利用马来布鲁线虫在沙鼠（长爪沙鼠）中进行杀成虫剂实验室评估的基础研究。2. 一种杀成虫剂评估新方法的建立与评价。
Basic studies on the laboratory assessment of macrofilaricides using Brugia malayi in the jird, Meriones unguiculatus. 2. Establishment and evaluation of a new method of macrofilaricide assessment.
作者信息
Maeda R, Hayashi Y, Shibuya T
机构信息
Department of Parasitology, Teikyo University School of Medicine, Tokyo, Japan.
出版信息
Jpn J Exp Med. 1988 Feb;58(1):45-9.
Jird infected subcutaneously with infective stage larvae (L3) of Brugia malayi were evaluated as an animal model for assessing macrofilaricides using a method of observing the change in microfilaria (mf) density but not by recovering adult worms. The animals were treated with a test compound followed by diethylcarbamazine (DEC) at 50 mg/kg for 5 consecutive days for clearing the existing mf from the blood stream. A continuous decrease in mf density was observed when jirds were treated with flubendazole. Nevertheless, slow recovery of mf density was observed in the jirds which were given suramin or Mel W, indicating that mf productivity of female worms was continuing after DEC treatment. The results obtained by monitoring microfilaremia corresponded with those obtained by recovery of adult worms at autopsy, suggesting that the system of L3-induced B. malayi jird model is useful for testing macrofilaricides.
将感染马来布鲁线虫感染期幼虫（L3）的沙鼠作为动物模型，采用观察微丝蚴（mf）密度变化的方法而非回收成虫的方法来评估杀成虫剂。给动物施用受试化合物，随后连续5天以50 mg/kg的剂量施用乙胺嗪（DEC），以清除血流中现有的mf。用氟苯达唑处理沙鼠时，观察到mf密度持续下降。然而，在给予苏拉明或Mel W的沙鼠中观察到mf密度缓慢恢复，这表明DEC处理后雌虫的mf产生能力仍在继续。监测微丝蚴血症获得的结果与尸检时回收成虫获得的结果一致，表明L3诱导的马来布鲁线虫沙鼠模型系统可用于测试杀成虫剂。
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