Department of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Ondokuz Mayıs University, 55139 Samsun, Turkey.
Department of Microbiology, Oregon State University, 97331 Corvallis, OR, USA.
Parasitol Int. 2021 Aug;83:102339. doi: 10.1016/j.parint.2021.102339. Epub 2021 Mar 30.
Turkey has more than 200 endemic freshwater fish species, one of which is the Ankara nase, Chondrostoma angorense Elvira, 1987 (Cypriniformes: Leuciscidae), a food fish in northern Turkey. Like most endemic fish species in Turkey, its myxosporean parasite fauna (Cnidaria: Myxosporea) are not yet described. We surveyed twenty C. angorense from Lâdik Lake in northern Turkey, and identified two myxosporean parasites from gills of these fish: Myxobolus arrabonensis Cech, Borzák, Molnár, Székely, 2015, and a co-infection of a novel species, Myxobolus polati sp. nov. We characterized both infections based on myxospore morphology, morphometry, tissue tropism, small subunit ribosomal DNA sequence and phylogenetic analysis. Plasmodia of both species were observed in gills, but had distinct tropism: M. arrabonensis is an intrafilamental vascular type, and M. polati sp. nov. is an intralamellar vascular type. We identified M. arrabonensis on the basis of myxospore characters and 100% similarity to the type DNA sequence from the closely-related host C. nasus. The small subunit ribosomal DNA sequence of M. polati sp. nov. (1946 base pairs; GenBank Accession number MH392318) had a maximum similarity of 98% with any Myxobolus sp. from other Eurasian cypriniforms. Phylogenetic analysis revealed that M. polati sp. nov. is most closely related to gill-infecting Myxobolus diversicapsularis from Rutilus rutilus (L.). The present study is the first record of myxosporean species infecting C. angorense comprising a novel species, M. polati sp. nov. and a known species M. arrabonensis.
土耳其拥有超过 200 种特有淡水鱼类，其中一种是安卡拉泥鳅，Chondrostoma angorense Elvira, 1987（鲤形目：Leuciscidae），是土耳其北部的一种食用鱼类。与土耳其大多数特有鱼类一样，其粘孢子虫寄生虫区系（刺胞动物门：粘孢子虫）尚未描述。我们调查了来自土耳其北部 Lâdik 湖的 20 条 C. angorense，并从这些鱼的鳃中鉴定出两种粘孢子虫寄生虫：Myxobolus arrabonensis Cech, Borzák, Molnár, Székely, 2015，以及一种新种的合并感染，Myxobolus polati sp. nov.。我们根据粘孢子虫形态、形态测量学、组织嗜性、小亚基核糖体 DNA 序列和系统发育分析对这两种感染进行了特征描述。两种物种的质体都在鳃中观察到，但具有不同的嗜性：M. arrabonensis 是一种内纤维血管型，而 M. polati sp. nov. 是一种内板血管型。我们根据粘孢子虫特征和与密切相关宿主 C. nasus 的 100%相似性鉴定出 M. arrabonensis。M. polati sp. nov. 的小亚基核糖体 DNA 序列（1946 个碱基对；GenBank 注册号 MH392318）与欧亚鲤形目其他鱼类的任何 Myxobolus sp. 的相似度最高为 98%。系统发育分析表明，M. polati sp. nov. 与鳃感染的 Myxobolus diversicapsularis 来自 Rutilus rutilus (L.) 最为密切相关。本研究首次记录了感染 C. angorense 的粘孢子虫物种，包括一种新种 M. polati sp. nov. 和一种已知种 M. arrabonensis。
