Kaur Harpreet, Katoch Anu
Department of Zoology, Panjab University, 160014, Chandigarh, India.
Dis Aquat Organ. 2016 Feb 25;118(2):129-37. doi: 10.3354/dao02959.
Native carp species cultured in Indian farms in Punjab (catla Catla catla, rohu Labeo rohita, mrigal Cirrhinus mrigala, exotic carps such as silver carp Hypophthalmichthys molitrix, grass carp Ctenopharyngodon idella, common carp Cyprinus carpio and a catfish Sperata seenghala) were examined for the presence of myxozoan parasites infecting gills. Firstly, the gills were examined under a zoom-stereomicroscope for the presence of plasmodia. The number of plasmodia per gill was counted to determine the index for the intensity of infection. Infected tissues were processed for histology, and 3-4 µm sections of infected gills were stained with haematoxylin & eosin and Luna's method. A total of 19 species of myxosporean were found infecting various cell types in the gills. Of these, 14 species belonged to the genus Myxobolus, 3 species to the genus Thelohanellus and 2 species to the genus Henneguya. Species belonging to the genus Myxobolus formed the interlamellar and intralamellar vascular (LV) type plasmodia, and species belonging to the genus Thelohanellus and Henneguya formed intrafilamental vascular (FV) type plasmodia. Mixed infections comprising 2, 3 or 4 different myxozoan species were noted in individual fish. The most common type of parasitism was polyparasitism due to 4 myxobolids co-occuring in fish with an infection rate of 23.16%. All species caused mild to severe haemorrhagic gill disease with little clinical symptomatology.
对旁遮普邦印度养殖场养殖的本地鲤鱼品种（印度鲃、露斯塔野鲮、麦瑞加拉鲮、外来鲤鱼如鲢鳙、草鱼、鲤鱼以及一种鲶鱼）的鳃部进行检查，以确定是否存在感染鳃部的粘孢子虫寄生虫。首先，在体视显微镜下检查鳃部是否存在孢囊。计算每片鳃上孢囊的数量，以确定感染强度指数。对感染组织进行组织学处理，用苏木精和伊红以及卢娜氏法对感染鳃部的3 - 4微米切片进行染色。共发现19种粘孢子虫感染鳃部的各种细胞类型。其中，14种属于粘体虫属，3种属于泰勒虫属，2种属于尾孢虫属。粘体虫属的物种形成片层间和片层内血管（LV）型孢囊，泰勒虫属和尾孢虫属的物种形成丝内血管（FV）型孢囊。在个体鱼中发现了由2、3或4种不同粘孢子虫物种组成的混合感染。最常见的寄生类型是多寄生，即4种粘体虫同时出现在鱼体中，感染率为23.16%。所有物种均引发了轻度至重度的出血性鳃病，且几乎没有临床症状。
