Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box -2455, Riyadh, 11451, Saudi Arabia.
Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box -2455, Riyadh, 11451, Saudi Arabia.
Photodiagnosis Photodyn Ther. 2021 Jun;34:102276. doi: 10.1016/j.pdpdt.2021.102276. Epub 2021 Mar 30.
Spectral diagnostic screening for sickle cell disease was carried out on volunteer blood samples (N = 100). The samples were subjected to different diagnostic methods including conventional complete blood count (CBC), hemoglobin electrophoresis (HBE) and spectral diagnosis. For the spectral diagnostic method, we discriminated three different characteristic spectral features. In total, 15 samples were sickle cell trait (SCT), 34 samples were sickle cell disease (SCD), and the rest of the samples (N = 51) were normal controls. The spectral discrimination of the three different sets of samples was distinguished on the quantification of fluorescent biomolecules such as tyrosine, tryptophan, NADH, FAD, and porphyrins. The results were compared with the conventional standard CBC and capillary electrophoresis findings. The spectral diagnosis method exhibited a sensitivity and specificity greater than 90 % for the tested samples. This technique requires only 5 mL blood samples, has an analysis time of 20 min, exhibits high accuracy and may be used in small clinics in remote villages.
对志愿者的血液样本（N=100）进行了镰状细胞病的光谱诊断筛查。这些样本采用了不同的诊断方法，包括常规全血细胞计数（CBC）、血红蛋白电泳（HBE）和光谱诊断。对于光谱诊断方法，我们区分了三种不同的特征光谱特征。总共，有 15 个样本是镰状细胞特征（SCT），34 个样本是镰状细胞病（SCD），其余的样本（N=51）是正常对照。通过定量分析色氨酸、色氨酸、NADH、FAD 和卟啉等荧光生物分子来区分这三组不同样本的光谱差异。将结果与传统的标准 CBC 和毛细管电泳结果进行比较。光谱诊断方法对测试样本的灵敏度和特异性均大于 90%。该技术仅需 5mL 血液样本，分析时间为 20 分钟，具有较高的准确性，可在偏远村庄的小型诊所使用。
