Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia.
Research Chair in Laser Diagnosis of Cancers, Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, Saudi Arabia.
Dis Markers. 2018 Aug 27;2018:5942368. doi: 10.1155/2018/5942368. eCollection 2018.
Sickle cell anemia (SCA) is an inherited blood disorder with worldwide incidence of 15%; out of this, it is found in up to 20% in countries like Kingdom of Saudi Arabia and Bahrain. The standard conventional method of detection is complete blood count (CBC) followed by hemoglobin electrophoresis or high-performance liquid chromatography (HPLC) or both. In this context, spectral detection of variants of sickle cell anemia (SCA) is an innovative technique, which when made accurate and reliable could be an effective alternative, since the instrumentation is compact (5 kg) and hence portable. This makes mass screening even in remote villages possible. In this paper, we give the essential aspects of fluorescent spectral features of sickle cell trait (SCT), sickle cell disease (SCD), beta () thalassemia trait (BTT) + SCD, and beta () thalassemia disease (BTD) + SCD. All the above four major variants could be discriminated among themselves and also from the normal control blood sample. All these analyses could be carried out with 5 ml of blood, in a time period of 10 minutes. The results of this paper give strong support for an alternative method, a spectral technique, for molecular-level diagnosis of sickle cell anemia and other closely related blood disorders.
镰状细胞贫血症（SCA）是一种具有全球发病率 15%的遗传性血液疾病；其中，在沙特阿拉伯和巴林等国家，发病率高达 20%。标准的常规检测方法是全血细胞计数（CBC），然后进行血红蛋白电泳或高效液相色谱（HPLC）或两者兼用。在这种情况下，镰状细胞贫血症（SCA）变体的光谱检测是一种创新技术，如果能够做到准确可靠，就可以成为一种有效的替代方法，因为该仪器小巧（5 公斤），因此便于携带。这使得即使在偏远村庄也可以进行大规模筛查。在本文中，我们给出了镰状细胞特征（SCT）、镰状细胞病（SCD）、β地中海贫血特征（BTT）+SCD 和β地中海贫血病（BTD）+SCD 的荧光光谱特征的基本方面。所有上述四种主要变体都可以相互区分，也可以与正常对照血液样本区分开来。所有这些分析都可以在 10 分钟的时间内使用 5 毫升血液进行。本文的结果为镰状细胞贫血症和其他密切相关的血液疾病的分子水平诊断提供了强有力的支持，即光谱技术是一种替代方法。
