Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan; Department of Orthodontics, School of Dentistry, Showa University, Tokyo, Japan.
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
Biochem Biophys Res Commun. 2021 May 21;554:173-178. doi: 10.1016/j.bbrc.2021.03.079. Epub 2021 Mar 30.
Neural crest-derived cells (NCDCs), a class of adult stem cells not restricted to embryonic tissues, are attractive tissue regenerative therapy candidates because of their ease of isolation, self-renewing properties, and multipotency. Although adult NCDCs can undergo osteogenic differentiation in vitro, whether they induce bone formation in vivo remains unclear. Previously, our group reported findings showing high amounts of NCDCs scattered throughout nasal concha tissues of adult mice. In the present study, NCDCs in nasal conchae labeled with enhanced green fluorescent protein (EGFP) were collected from adult P0-Cre/CAG-CAT-EGFP double transgenic mice, then cultured in serum-free medium to increase the number. Subsequently, NCDCs were harvested and suspended in type I atelocollagen gel, then an atelocollagen sponge was used as a scaffold for the cell suspension. Atelocollagen scaffolds with NCDCs were placed on bone defects created in a mouse calvarial bone defect model. Over the ensuing 12 weeks, micro-CT and histological analysis findings showed that mice with scaffolds containing NCDCs had slightly greater bone formation as compared to those with a scaffold alone. Furthermore, Raman spectroscopy revealed spectral properties of bone in mice that received scaffolds with NCDCs similar to those of native calvarial bone. Bone regeneration is important not only for gaining bone mass but also chemical properties. These results are the first to show the validity of biomolecule-free adult nasal concha-derived NCDCs for bone regeneration, including the chemical properties of regenerated bone tissue.
神经嵴衍生细胞（NCDCs）是一类不限于胚胎组织的成人干细胞，由于其易于分离、自我更新特性和多能性，成为有吸引力的组织再生治疗候选物。尽管成人 NCDCs 可以在体外进行成骨分化，但它们是否能在体内诱导骨形成尚不清楚。先前，我们的研究小组报告了在成年小鼠的鼻甲组织中发现大量散在分布的 NCDCs 的发现。在本研究中，从成年 P0-Cre/CAG-CAT-EGFP 双转基因小鼠的鼻甲中收集标记有增强型绿色荧光蛋白（EGFP）的 NCDCs，然后在无血清培养基中培养以增加数量。随后，收集 NCDCs 并悬浮在 I 型原胶原凝胶中，然后使用原胶原海绵作为细胞悬浮液的支架。将含有 NCDCs 的原胶原支架放置在小鼠颅骨骨缺损模型中创建的骨缺损上。在接下来的 12 周内，通过微 CT 和组织学分析发现，与仅使用支架的小鼠相比，含有 NCDCs 支架的小鼠的骨形成略有增加。此外，拉曼光谱显示，接受含有 NCDCs 支架的小鼠的骨具有与天然颅骨骨相似的光谱特性。骨再生不仅对获得骨量很重要，而且对化学性质也很重要。这些结果首次表明，无生物分子的成人鼻腔衍生 NCDCs 对骨再生的有效性，包括再生骨组织的化学性质。
