Biosciences and Food Technology, School of Science, RMIT University, Melbourne, Australia.
Biosciences and Food Technology, School of Science, RMIT University, Melbourne, Australia.
Meat Sci. 2021 Jul;177:108508. doi: 10.1016/j.meatsci.2021.108508. Epub 2021 Mar 22.
The focus of this study was to compare the effectiveness of MALDI-TOF MS and partial 16S rRNA gene sequencing for the identification of bacteria isolated from VP lamb meat stored chilled at 5 °C for 21 days, at the same time gaining insights into bacterial changes over time. The identity of bacterial isolates on non-selective and selective agars was determined by both methods and results compared. Results showed that total bacterial numbers increased over the 21 days (as expected) with Staphylococcus and Pseudomonas (day 0) being replaced by Carnobacterium, Brochothrix and members of the Enterobacteriaceae family by day 21. A high level of agreement (86-100%) for bacterial isolates' identity at genus level was observed between MALDI-TOF MS and partial 16S rRNA gene-based sequencing for isolates where identification was possible. With its cheaper cost and faster turnaround time, once optimized, MALDI-TOF MS could become a useful alternative to 16S rRNA gene-sequencing for the rapid identification of red meat bacterial isolates.
本研究的重点是比较 MALDI-TOF MS 和部分 16S rRNA 基因测序在鉴定冷藏（5°C）21 天后 VP 羊肉中分离出的细菌的有效性，同时深入了解细菌随时间的变化。通过这两种方法确定非选择性和选择性琼脂上细菌分离物的身份，并对结果进行比较。结果表明，总细菌数量在 21 天内增加（预期），金黄色葡萄球菌和假单胞菌（第 0 天）被肉杆菌属、短杆菌属和肠杆菌科的成员取代。对于可鉴定的分离物，MALDI-TOF MS 和基于部分 16S rRNA 基因测序在属水平上对细菌分离物的鉴定具有高度一致性（86-100%）。MALDI-TOF MS 成本更低，周转时间更快，一旦优化，它可能成为快速鉴定红肉细菌分离物的 16S rRNA 基因测序的替代方法。
