Department of Molecular Biology and Biotechnology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture and Technology, Udaipur, Rajasthan, 313 001, India.
Department of Biochemistry, College of Basic Sciences and Humanities, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana, 125 004, India.
Plant Physiol Biochem. 2021 May;162:699-705. doi: 10.1016/j.plaphy.2021.03.021. Epub 2021 Mar 15.
Sustained source-activity is imperative for vigor plant growth and yield. In present study, physio-biochemical responses of wheat plant contributing to source-activity were measured after application of salicylic acid-chitosan nanoparticles (SA-CS NPs). SA-CS NPs slowly release SA for sustained availability to plant. In seedling bioassay, as compared with salicylic acid (SA), SA-CS NPs incurred up to ~1.5 folds increased activities of seed reserve food remobilizing enzymes for substantial mobilization of reserve food to growing seedlings and enhanced seedling vigor index (SVI) by 1.6 folds. At booting stage, foliar application of SA-CS NPs (0.01-0.08%; w/v) enhanced the activities of superoxide dismutase (1.94 folds), catalase (1.33 folds), peroxidase (1.99 folds) and polyphenol oxidase (1.04 folds) in flag leaf. SA-CS NPs further contrived cellular homeostasis by comforting reactive oxygen species (ROS), malondialdehyde (MDA) and proline contents in flag leaf. SA-CS NPs (0.08%; w/v) significantly increased chlorophylls (a-b) contents (1.46 folds), spike length without awns, spike lets per spike and grain weight per pot as compared with SA. Study categorically explicates that slow release of SA from SA-CS NPs could exert significant effect on source-activity by maneuvering various physio-biochemical responses of wheat plant.
持续的源活性对作物生长和产量至关重要。在本研究中，在施用水杨酸-壳聚糖纳米粒子（SA-CS NPs）后，测量了小麦植株对源活性有贡献的生理生化反应。SA-CS NPs 缓慢释放 SA，以维持其在植物中的可用性。在幼苗生物测定中，与水杨酸（SA）相比，SA-CS NPs 使种子储备食物再动员酶的活性增加了约 1.5 倍，从而将储备食物大量动员到生长中的幼苗中，并使幼苗活力指数（SVI）提高了 1.6 倍。在拔节期，叶面喷施 SA-CS NPs（0.01-0.08%，w/v）可提高旗叶中超氧化物歧化酶（1.94 倍）、过氧化氢酶（1.33 倍）、过氧化物酶（1.99 倍）和多酚氧化酶（1.04 倍）的活性。SA-CS NPs 通过缓解旗叶中的活性氧（ROS）、丙二醛（MDA）和脯氨酸含量，进一步维持细胞内稳态。与 SA 相比，SA-CS NPs（0.08%，w/v）显著增加了叶绿素（a-b）含量（1.46 倍）、无芒穗长、穗粒数和每盆粒重。研究明确表明，SA-CS NPs 从 SA 中的缓慢释放可以通过操纵小麦植株的各种生理生化反应对源活性产生显著影响。
