壳聚糖纳米粒子对小麦（Triticum aestivum L.）种子萌发和幼苗生长的影响。

Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.).

机构信息

Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; School of Environment Science and Engineering, Dalian Maritime University, Dalian 116026, China.

Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; School of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China.

出版信息

Int J Biol Macromol. 2019 Apr 1;126:91-100. doi: 10.1016/j.ijbiomac.2018.12.118. Epub 2018 Dec 14.

DOI:10.1016/j.ijbiomac.2018.12.118

PMID:30557637

Abstract

To investigate the effect and mechanism of chitosan nanoparticles (CSNPs) on the germination and seedling growth of wheat (Triticum aestivum L.), we conducted systematic research on the impact of different concentrations (1-100 μg/mL) of CSNPs and chitosan (CS). The result of energy-dispersive spectroscopy (EDS) and confocal laser scanning microscopy (CLSM) showed that adsorption of CSNPs on the surface of wheat seeds was higher than that of CS. CSNPs had growth promoting effect at a lower concentration (5 μg/mL) compared with CS (50 μg/mL). In addition, the application of 5 μg/mL CSNPs induced the auxin-related gene expression, accelerated indole-3-acetic acid (IAA) biosynthesis and transport, and reduced IAA oxidase activity resulting in the increase of IAA concentration in wheat shoots and roots. The results suggest that CSNPs have positive effect on seed germination and seedling growth of wheat at a lower concentration than CS due to higher adsorption on the surface of wheat seeds.

摘要

为了研究壳聚糖纳米粒子（CSNPs）对小麦（Triticum aestivum L.）萌发和幼苗生长的影响及其作用机制，我们系统研究了不同浓度（1-100μg/mL）的 CSNPs 和壳聚糖（CS）对小麦种子的影响。能谱分析（EDS）和共聚焦激光扫描显微镜（CLSM）的结果表明，CSNPs 在小麦种子表面的吸附率高于 CS。与 CS（50μg/mL）相比，CSNPs 在较低浓度（5μg/mL）下具有生长促进作用。此外，应用 5μg/mL CSNPs 诱导了与生长素相关的基因表达，加速了吲哚-3-乙酸（IAA）的生物合成和运输，并降低了 IAA 氧化酶的活性，导致小麦地上部和根部的 IAA 浓度增加。结果表明，CSNPs 由于在小麦种子表面具有更高的吸附率，在较低浓度下对小麦种子萌发和幼苗生长具有积极作用。