Bogusz M, Aderjan R
Institute of Forensic Medicine, Karl-Ruprechts-University, Heidelberg, Federal Republic of Germany.
J Anal Toxicol. 1988 Mar-Apr;12(2):67-72. doi: 10.1093/jat/12.2.67.
Thirty-seven selected acidic and neutral drugs were analyzed by HPLC with two-step gradient elution and nine different ODS silica columns. The retention index values (RI) for drugs were calculated against a series of alkyl-arylketones and corrected according to the procedure described earlier. The correction procedure decreased the intercolumn variability of the RI values, and the differences between the RI values obtained for the reference column and other columns substantially declined after correction. The mean standard deviation of uncorrected RI values, calculated for all drugs analyzed on 9 columns, amounted to 25 RI units (range 8 to 40). After correction, the mean standard deviation was 15 RI units (range 3 to 26). The method proved to be suitable for the comparison of results obtained with commercially different ODS silica columns.
采用两步梯度洗脱法，使用9种不同的ODS硅胶柱，通过高效液相色谱法对37种选定的酸性和中性药物进行了分析。根据一系列烷基芳基酮计算药物的保留指数值（RI），并按照先前描述的程序进行校正。校正程序降低了RI值的柱间变异性，校正后，参比柱与其他柱所获得的RI值之间的差异大幅下降。对9根柱子上分析的所有药物计算得到的未校正RI值的平均标准差为25 RI单位（范围为8至40）。校正后，平均标准差为15 RI单位（范围为3至26）。该方法被证明适用于比较不同商业ODS硅胶柱获得的结果。
