Bogusz M, Neidl-Fischer G, Aderjan R
Institute of Forensic Medicine, Karl-Ruprecht University, Heidelberg, Federal Republic of Germany.
J Anal Toxicol. 1988 Nov-Dec;12(6):325-9. doi: 10.1093/jat/12.6.325.
Sixteen basic drugs were examined by HPLC (gradient elution in acetonitrile/phosphate buffer, pH 3.2, containing 0.05% nonylamine) with six different ODS-silica columns. The retention indices (RI) were calculated with alkyl arylketone and 1-nitroalkane scales and were subjected to correction, which enabled comparison of results from commercially different column packing materials. The correction procedure was successful for the 1-nitroalkane scale. The scale based on alkyl arylketones was of less use for basic drugs, because some of them eluted earlier than the first reference homologue. This made impossible the proper calculation and correction of RI values for drugs such as cocaine, diphenhydramine, doxepin, and promethazine. The correction procedure of RI values calculated against the series of 1-nitroalkanes is recommended as a method of standardization of HPLC data.
使用六种不同的十八烷基硅烷键合硅胶柱，通过高效液相色谱法（在乙腈/磷酸盐缓冲液，pH 3.2，含0.05%壬胺中进行梯度洗脱）对16种碱性药物进行了检测。保留指数（RI）采用烷基芳基酮和1-硝基烷烃标度进行计算，并进行了校正，从而能够比较来自商业上不同柱填充材料的结果。校正程序对1-硝基烷烃标度是成功的。基于烷基芳基酮的标度对碱性药物的用途较小，因为其中一些药物的洗脱时间比第一个参考同系物更早。这使得可卡因、苯海拉明、多塞平、异丙嗪等药物的RI值无法进行正确的计算和校正。建议采用针对1-硝基烷烃系列计算的RI值校正程序作为高效液相色谱数据标准化的一种方法。
