Bogusz M, Erkens M
Institute of Forensic Medicine, Medical Faculty, Aachen University of Technology, Germany.
J Anal Toxicol. 1995 Jan-Feb;19(1):49-55. doi: 10.1093/jat/19.1.49.
Human serum, autopsy blood, and liver samples were extracted by means of liquid-liquid extraction at acidic and at basic pH and at basic pH following acid hydrolysis. Six selected basic and six selected acidic or neutral drugs were added to the extracts. The spiked and blank samples were examined by means of reversed-phase high-performance liquid chromatography (HPLC) under standardized conditions using diode array detection. The retention index (RI) values of drugs were determined using a 1-nitroalkane scale and secondary standards. The RI values were virtually unaffected by biological matrix. On the other hand, the detection of some substances may be impaired by matrix peaks occurring regularly in biological extracts. The retention index values and UV spectra of three identified (phenethylamine, tryptamine, and indole) and nine unidentified substances were included in an HPLC database.
通过在酸性和碱性pH条件下以及酸水解后在碱性pH条件下进行液-液萃取，从人血清、尸检血液和肝脏样本中提取物质。向提取物中添加六种选定的碱性药物以及六种选定的酸性或中性药物。加标样本和空白样本在标准化条件下采用二极管阵列检测的反相高效液相色谱法（HPLC）进行检测。使用1-硝基烷烃标度和二级标准物测定药物的保留指数（RI）值。RI值实际上不受生物基质的影响。另一方面，生物提取物中经常出现的基质峰可能会影响某些物质的检测。三种已鉴定物质（苯乙胺、色胺和吲哚）以及九种未鉴定物质的保留指数值和紫外光谱被纳入HPLC数据库。
