一种采用火焰离子化检测的薄层色谱法快速灵敏测定血浆和尿液中百草枯的方法。

A rapid and sensitive method for the determination of paraquat in plasma and urine by thin-layer chromatography with flame ionization detection.

作者信息

Ikebuchi J, Yuasa I, Kotoku S

机构信息

Department of Legal Medicine, Tottori University School of Medicine, Yonago, Japan.

出版信息

J Anal Toxicol. 1988 Mar-Apr;12(2):80-3. doi: 10.1093/jat/12.2.80.

DOI:10.1093/jat/12.2.80

PMID:3379926

Abstract

A new method for the determination of paraquat in biological fluids has been developed. It involves thin-layer chromatography with flame ionization detection (TLC/FID) and simple solid-phase extraction with a disposable octadecylsilane column. This procedure allows direct loading of the samples on the disposable column. When Chromarod SII is used as the stationary phase and methanol-2N hydrochloric acid (2:3, v/v) as the mobile phase, recovery levels of paraquat in spiked materials are from 87.5 to 98.0%. This method can be used for samples of paraquat at levels as low as 50 ng.

摘要

已开发出一种测定生物体液中百草枯的新方法。该方法包括采用火焰离子化检测的薄层色谱法（TLC/FID）和使用一次性十八烷基硅烷柱的简单固相萃取法。此程序允许将样品直接加载到一次性柱上。当使用Chromarod SII作为固定相，甲醇 - 2N盐酸（2:3，v/v）作为流动相时，加标物质中百草枯的回收率为87.5%至98.0%。该方法可用于低至50 ng的百草枯样品。