Institute of Food Science, Technology and Nutrition (CSIC), 10 José Antonio Novais St, 28040 Madrid, Spain.
Department of Food Science and Human Nutrition, School of Food and Nutritional Sciences, Agricultural University of Athens, Iera Odos 75, Athens, GR 11855, Greece.
Food Chem. 2021 Sep 1;355:129649. doi: 10.1016/j.foodchem.2021.129649. Epub 2021 Mar 23.
The market value of crustaceans depreciates during storage due to the appearance of melanosis caused by polyphenol oxidases. Sulfite derivatives are used as melanosis-inhibiting agents, but their unhealthy effects make it preferable to replace them with natural preservatives. In this work, a crude enzymatic extract from whiteleg shrimp (Penaeus vannamei) was characterized and used to test the diphenol oxidase-inhibiting activity of polyphenol extracts of five underutilized halophyte plants, namely crystalline ice plant, seaside arrowgrass, purslane, sea fennel, and seashore aster. The extracts inhibited diphenol oxidase activity more efficiently than sodium sulfite. The purslane extract was rich in isoorientins, isovitexin, and apigenin, and showed the highest inhibiting effect, being this classified as mixed or non-competitive. Hydroxyl groups in the phenyl B ring could be responsible for the inhibitory activity of the extract. The polyphenol extracts tested in this work could be promising melanosis-inhibiting agents of interest for seafood industries.
甲壳类动物在储存过程中由于多酚氧化酶引起的黑色素沉着而导致市场价值贬值。亚硫酸盐衍生物被用作黑色素抑制剂，但由于它们的不健康影响，最好用天然防腐剂代替它们。在这项工作中， characterization 从白对虾（Penaeus vannamei）中提取了一种粗酶提取物，并用于测试五种未充分利用的盐生植物的多酚提取物对二酚氧化酶的抑制活性，分别为冰晶菜、海滨雀稗、马齿苋、海茴香和海滨 Aster。提取物对二酚氧化酶的抑制活性高于亚硫酸钠。马齿苋提取物富含异牡荆素、异荭草素和芹菜素，表现出最高的抑制效果，被归类为混合或非竞争性。苯 B 环上的羟基基团可能是提取物具有抑制活性的原因。本工作中测试的多酚提取物可能是有前途的海鲜工业用黑色素抑制剂。
