Hietter H, Gouyette A, Van Dorsselaer A, Luu B
Laboratoire de Chimie Organique des Substances Naturelles associé au CNRS, Centre de Neurochimie, Strasbourg, France.
J Steroid Biochem. 1988 May;29(5):481-9. doi: 10.1016/0022-4731(88)90182-3.
The metabolism of labelled 7 alpha- and 7 beta-hydroxycholesterol was investigated in two lymphoma cell lines (YAC-1, RDM-4), in murine splenocytes and in HTC hepatoma cells. The structures of the metabolites in lymphoma cells were determined as 3 beta-esters of C14-C20 fatty acids by nuclear magnetic resonance and mass spectrometric studies. In hepatoma cells, more polar metabolites of 7 alpha- and 7 beta-hydroxycholesterol were detected whereas, in non-dividing lymphocyte cells, no metabolic transformation occurs. Therefore, metabolic transformation of the hydroxycholesterol is not required for the expression of their activity and the question of the physiological role of the metabolic products is raised.
在两种淋巴瘤细胞系（YAC - 1、RDM - 4）、小鼠脾细胞和HTC肝癌细胞中研究了标记的7α - 和7β - 羟基胆固醇的代谢情况。通过核磁共振和质谱研究确定淋巴瘤细胞中代谢产物的结构为C14 - C20脂肪酸的3β - 酯。在肝癌细胞中，检测到了7α - 和7β - 羟基胆固醇的极性更强的代谢产物，而在不分裂的淋巴细胞中，未发生代谢转化。因此，羟基胆固醇的活性表达并不需要其进行代谢转化，这就引出了代谢产物的生理作用问题。
