Stephens R, Alitalo R, Tapiovaara H, Vaheri A
Department of Virology, University of Helsinki, Finland.
Leuk Res. 1988;12(5):419-22. doi: 10.1016/0145-2126(88)90061-6.
A new screening test is described which enabled rapid determination of the proportion of single-chain and two-chain urokinase produced in the culture supernatants of 18 human cell lines. A clear distinction was found between two groups of cell lines: cells derived from ten solid tumors produced almost exclusively single-chain proenzyme, while the majority of the enzyme found in cultures of eight leukemia cell lines was in the active, two-chain form.
本文描述了一种新的筛选试验，该试验能够快速测定18种人类细胞系培养上清液中产生的单链和双链尿激酶的比例。在两组细胞系之间发现了明显的差异：来自10种实体瘤的细胞几乎只产生单链酶原，而在8种白血病细胞系培养物中发现的大多数酶是活性双链形式。
