使用薰衣草和罗勒精油作为木材生物杀菌剂的新方法：气相色谱-质谱联用、扫描电子显微镜和MNDO量子化学研究

New Approach for Using of L. and L. Essential Oils as Wood-Biofungicides: GC-MS, SEM, and MNDO Quantum Chemical Studies.

作者信息

Ali Hayssam M, Elgat Wael A A Abo, El-Hefny Mervat, Salem Mohamed Z M, Taha Ayman S, Al Farraj Dunia A, Elshikh Mohamed S, Hatamleh Ashraf A, Abdel-Salam Eslam M

机构信息

Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.

Agriculture Research Center, Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Alexandria 21526, Egypt.

出版信息

Materials (Basel). 2021 Mar 11;14(6):1361. doi: 10.3390/ma14061361.

BACKGROUND

Fungi growing on wood cause deterioration of stored food materials or discoloration of the wood itself, and the search for new and safe bioagents is recently needed.

METHODS

Essential oils (EOs) from aerial parts from L. and L., analyzed by gas chromatography-mass spectrometry (GC-MS), were tested for their antifungal activity by the vapor method against four common fungi, , , , and , and confirmed by SEM examination as the oils applied on wood samples.

RESULTS

The most abundant compounds identified in the EO from were menthone and eucalyptol; in EO, they were β-caryophyllene, β-caryophyllene oxide, and β-elemene. EOs from and , at 500 and 250 µL/mL, showed potent antifungal activity against and , with 100% fungal mycelial inhibition growth (FMIG). and EOs, at 125 µL/mL, observed FMIG values of 98% and 95%, respectively, against . EO, at 500 and 250 µL/mL, showed potent activity against , with 100% FMIG. completely inhibited (100% FMIG) EOs from and applied at 500 µL/mL. Sarg. Wood, treated with at 125 µL/mL, showed inhibition zone values of 7.33 and 21.33 mm against and , respectively.

CONCLUSIONS

Both oils possessed good wood-biofungicide activity with the vapor method, as clearly shown by the SEM examination. These activities suggest their possible use as natural wood preservatives.

背景

生长在木材上的真菌会导致储存的食品材料变质或木材本身变色，因此最近需要寻找新的安全生物制剂。

方法

通过气相色谱 - 质谱联用（GC - MS）分析了来自L.和L.地上部分的挥发油（EOs），采用气相法测试其对四种常见真菌，即，，，和的抗真菌活性，并通过扫描电子显微镜（SEM）检查对应用于木材样品上的油进行了确认。

结果

在EO中鉴定出的最丰富化合物是薄荷酮和桉叶油素；在EO中，它们是β - 石竹烯、β - 石竹烯氧化物和β - 榄香烯。来自和的EOs，在500和250μL/mL时，对和表现出强效抗真菌活性，真菌菌丝体生长抑制率（FMIG）为100%。和EOs，在125μL/mL时，对的FMIG值分别为98%和95%。EO，在500和250μL/mL时，对表现出强效活性，FMIG为100%。完全抑制了（100% FMIG）来自和的EOs在500μL/mL时的作用。用125μL/mL的处理的Sarg.木材，对和的抑菌圈值分别为7.33和21.33mm。