Ghazizadeh Mohammad, Davoodabadi Abolfazl, Kazemi Sohrab, Harandi Azadeh, Ghasempour Maryam
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
J Dent (Shiraz). 2025 Sep 1;26(3):266-273. doi: 10.30476/dentjods.2025.101488.2304. eCollection 2025 Sep.
() is a multidrug resistant pathogen that can cause persistent infections within the root canal system, which poses major challenges in dentistry and is involved in the majority of endodontic failures.
This investigation was conducted to evaluate the antibacterial properties of (M. longifolia) essential oil as a root canal irrigant against and its compounds.
In this study, 60 primary canines were divided into five groups: G1: Normal saline (negative control), G2: Normal saline, G3: 2.5% sodium hypochlorite (NaOCl), G4: 2% Chlorhexidine (CHX), and G5: M. longifolia essential oil based on minimum inhibitory concentration (MIC). The standard strain suspension of (ATCC 29212) was injected into the canal of all groups, except for the negative control group. After four weeks, the groups were washed with 2 ml of the introduced solutions and sampled by paper point to compare the antibacterial effect of these solutions. Broth microdilution method was used to determine the MIC, while Gas chromatography-mass spectrometry (GC/MS) was used to evaluate the chemical compositions of the essential oil. Data were analyzed based on one-way analysis of variance (ANOVA). Then, pairwise comparisons of groups were performed using Tukey's test.
The MIC of M. longifolia against was 10%. As a root canal irrigant, its highest antibacterial impact was associated with 2.5% NaOCl, 2% CHX, 10% essential oil, and normal saline. The difference was not statistically significant in antibacterial effect between 2.5% NaOCl and 2% CHX (p Value=0.64). However, the difference was statistically significant in antibacterial effect between 2.5% NaOCl and 10% M. longifolia, 2.5% NaOCl and normal saline, 2% CHX and 10% M. longifolia, 2% CHX and normal saline, and 10% M. longifolia and normal saline (< 0.001). Nineteen compounds were identified in the chemical analysis of M. longifolia, among which Piperitenone oxide (64.68%) and Piperitone oxide (23.68%) were the major compounds.
M. longifolia essential oil (10%) was effective against . The highest antibacterial effect of root canal irrigants was observed in 2.5% NaOCl, 2% CHX, 10% essential oil, and normal saline, respectively.
（某病原体名称未给出）是一种多重耐药病原体，可在根管系统内引起持续性感染，这给牙科带来了重大挑战，并且与大多数牙髓治疗失败有关。
本研究旨在评估长叶薄荷（M. longifolia）精油作为根管冲洗剂对（某病原体名称未给出）及其化合物的抗菌性能。
在本实验研究中，60颗乳牙犬齿被分为五组：G1：生理盐水（阴性对照组），G2：生理盐水，G3：2.5%次氯酸钠（NaOCl），G4：2%氯己定（CHX），以及G5：基于最低抑菌浓度（MIC）的长叶薄荷精油。除阴性对照组外，将（某病原体标准菌株名称未给出，ATCC 29212）的标准菌株悬液注入所有组的根管中。四周后，用2毫升引入的溶液冲洗各组，并用纸尖取样以比较这些溶液的抗菌效果。采用肉汤微量稀释法测定最低抑菌浓度（MIC），同时采用气相色谱 - 质谱联用（GC/MS）法评估精油的化学成分。数据基于单因素方差分析（ANOVA）进行分析。然后，使用Tukey检验对各组进行两两比较。
长叶薄荷对（某病原体名称未给出）的最低抑菌浓度为10%。作为根管冲洗剂，其最高抗菌效果与2.5% NaOCl、2% CHX、10%精油和生理盐水相关。2.5% NaOCl和2% CHX之间的抗菌效果差异无统计学意义（p值 = 0.64）。然而，2.5% NaOCl与10%长叶薄荷、2.5% NaOCl与生理盐水、2% CHX与10%长叶薄荷、2% CHX与生理盐水以及10%长叶薄荷与生理盐水之间的抗菌效果差异具有统计学意义（p < 0.001）。在长叶薄荷的化学分析中鉴定出19种化合物，其中氧化胡椒酮（64.68%）和氧化哌啶酮（23.68%）是主要化合物。
长叶薄荷精油（10%）对（某病原体名称未给出）有效。根管冲洗剂的最高抗菌效果分别在2.5% NaOCl、2% CHX、10%精油和生理盐水中观察到。
