González-Méndez R, Hahn G M, Wade-Jardetzky N G, Jardetzky O
Department of Therapeutic Radiology, Stanford University, California 94305-5055.
Magn Reson Med. 1988 Apr;6(4):373-80. doi: 10.1002/mrm.1910060402.
Intracellular pH measurements obtained by 31P NMR and DMO partitioning are compared. A continuous-flow culture system was used to measure the intracellular pH of Chinese hamster ovary fibroblasts in response to changes in the extracellular pH. These measurements were repeated with the CHO cells in suspension and under similar experimental conditions using the weak acid partitioning technique employing 14C-DMO. It is shown that the pHi values are identical, within experimental error, for both techniques in the 6.80-7.70 pH range, with the DMO technique giving slightly more acidic intracellular pH in the 6.00-6.80 range. It is concluded that both techniques give similar values for intracellular pH in the physiological range, with the DMO partitioning giving more acidic values at pH's less than 6.80. The range of validity for pHi measurements using 31P NMR lies between 6.70 and 7.50.
比较了通过31P核磁共振（NMR）和二甲基氧乙酸（DMO）分配法获得的细胞内pH测量结果。使用连续流动培养系统来测量中国仓鼠卵巢成纤维细胞在细胞外pH变化时的细胞内pH。在悬浮的CHO细胞中并在类似实验条件下，采用含14C-DMO的弱酸分配技术重复这些测量。结果表明，在6.80 - 7.70的pH范围内，两种技术测得的细胞内pH值（pHi）在实验误差范围内是相同的，而在6.00 - 6.80范围内，DMO技术测得的细胞内pH略呈酸性。得出的结论是，在生理范围内，两种技术给出的细胞内pH值相似，在pH小于6.80时，DMO分配法给出的pH值更呈酸性。使用31P NMR测量pHi的有效范围在6.70至7.50之间。
