GC-MS 验证法、GC-FID 和 HPLC-HRMS 对（Aubl.）Marchand 树脂中挥发性和非挥发性化合物的定性定量特征分析。

Quali-Quantitative Characterization of Volatile and Non-Volatile Compounds in (Aubl.) Marchand Resin by GC-MS Validated Method, GC-FID and HPLC-HRMS.

机构信息

Department of Molecular Biotechnology and Health Sciences, University of Torino, Via Pietro Giuria 5, 10125 Torino, Italy.

Biosfered S.r.l. Via Paolo Veronese 202, 10148 Turin, Italy.

出版信息

Molecules. 2021 Mar 7;26(5):1447. doi: 10.3390/molecules26051447.

DOI:10.3390/molecules26051447

PMID:33800018

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7962098/

Abstract

(Aubl.) Marchand (PH) trees are endemic to the tropical region of South America, mostly Brazil. Antibacterial, antinociceptive, anti-inflammatory, anxiolytic, antidepressant and anti-hyperlipidemic/anti-hypercholesterolemic effects were reported for its resinous exudate resin (PHR). This work aims to provide a qualitative and quantitative consistent chemical profiling of the major constituents of this resin and two extracts enriched in acid (acidic triterpene concentrated extract, ATCE) and neutral triterpenes (α and β-amyrin concentrated extract, AMCE). GC-MS/GC-FID was used for volatile terpene fraction, a validated GC-MS method was developed for quantification of neutral α and β-amyrin and HPLC-APCI HRMS was used for acidic triterpenes analysis. The chemical investigation reported 29 molecules, including 14 volatile terpenes, 6 neutral triterpenes and 11 acid triterpenes. The most abundant compounds were α-amyrin (251.28 g kg, 123.98 g kg and 556.82 g kg in PHR, ATCE and AMCE, respectively), β-amyrin (172.66 g kg, 95.39 g kg and 385.58 g kg in PHR, ATCE and AMCE, respectively), 3-oxo-tirucalla-7,24-dien-21-oic acid (80.64 g kg, 157.10 g kg and 15.31 g kg in PHR, ATCE and AMCE, respectively) and 3α-hydroxy-tirucalla-8,24-dien-21-oic acid (77.71 g kg, 130.40 g kg and 11.64 g kg in PHR, ATCE and AMCE, respectively). Results showed specific enrichment of acidic and neutral triterpenoids in the two respective extracts.

摘要

(Aubl.) Marchand (PH) 树原产于南美洲的热带地区，主要是巴西。其树脂状渗出物树脂 (PHR) 具有抗菌、镇痛、抗炎、抗焦虑、抗抑郁和抗高血脂/抗高胆固醇作用。本工作旨在对该树脂及其两种富含酸（酸性三萜浓缩提取物，ATCE）和中性三萜（α和β-香树脂醇浓缩提取物，AMCE）的提取物中的主要成分进行定性和定量一致的化学分析。GC-MS/GC-FID 用于挥发性萜烯馏分，建立了验证的 GC-MS 方法用于定量分析中性 α 和 β-香树脂醇，HPLC-APCI HRMS 用于酸性三萜分析。化学研究报告了 29 种分子，包括 14 种挥发性萜烯、6 种中性三萜和 11 种酸性三萜。最丰富的化合物是 α-香树脂醇（PHR、ATCE 和 AMCE 中的 251.28 g kg、172.66 g kg 和 556.82 g kg）、β-香树脂醇（PHR、ATCE 和 AMCE 中的 123.98 g kg、95.39 g kg 和 385.58 g kg）、3-氧代-齐墩果-7,24-二烯-21-酸（PHR、ATCE 和 AMCE 中的 80.64 g kg、157.10 g kg 和 15.31 g kg）和 3α-羟基-齐墩果-8,24-二烯-21-酸（PHR、ATCE 和 AMCE 中的 77.71 g kg、130.40 g kg 和 11.64 g kg）。结果表明，两种提取物中酸性和中性三萜类化合物有特定的富集。