Fellström Bengt, Helmersson-Karlqvist Johanna, Lind Lars, Soveri Inga, Thulin Måns, Ärnlöv Johan, Kultima Kim, Larsson Anders
Department of Medical Sciences, Uppsala University, Uppsala University Hospital, SE-751 85 Uppsala, Sweden.
Centre for Statistics, The Swedish University of Agricultural Sciences, SE-752 36 Uppsala, Sweden.
Biomolecules. 2021 Mar 8;11(3):396. doi: 10.3390/biom11030396.
The aim of the present study was to study the associations between urine albumin excretion, and a large number of urinary chemokines, cytokines, and growth factors in a normal population. We selected 90 urine samples from individuals without CVD, diabetes, stroke or kidney disease belonging to the Prospective Investigation of the Vasculature in Uppsala Seniors Study (41 males and 49 females, all aged 75 years). Urinary cytokine levels were analyzed with two multiplex assays (proximity extension assays) and the cytokine levels were correlated with urine albumin. After adjustment for sex, body mass index (BMI), estimated glomerular filtration rate (eGFR), smoking and multiplicity testing, 11 biomarkers remained significantly associated with urine albumin: thrombospondin 2, interleukin 6, interleukin 8, hepatocyte growth factor, matrix metalloproteinase-12 (MMP-12), C-X-C motif chemokine 9, tumor necrosis factor receptor superfamily member 11B, osteoprotegerin, growth-regulated alpha protein, C-X-C motif chemokine 6, oncostatin-M (OSM) and fatty acid-binding protein, intestinal, despite large differences in molecular weights. In this study, we found associations between urinary albumin and both small and large urine proteins. Additional studies are warranted to identify cytokine patterns and potential progression markers in various renal diseases.
本研究的目的是在正常人群中研究尿白蛋白排泄与大量尿趋化因子、细胞因子及生长因子之间的关联。我们从乌普萨拉老年血管前瞻性研究中选取了90份无心血管疾病、糖尿病、中风或肾病个体的尿液样本（41名男性和49名女性，均为75岁）。采用两种多重检测法（邻位延伸检测法）分析尿细胞因子水平，并将细胞因子水平与尿白蛋白进行关联分析。在对性别、体重指数（BMI）、估算肾小球滤过率（eGFR）、吸烟情况及多重检验进行校正后，11种生物标志物仍与尿白蛋白显著相关：血小板反应蛋白2、白细胞介素6、白细胞介素8、肝细胞生长因子、基质金属蛋白酶-12（MMP-12）、C-X-C基序趋化因子9、肿瘤坏死因子受体超家族成员11B、骨保护素、生长调节α蛋白、C-X-C基序趋化因子6、制瘤素-M（OSM）和肠脂肪酸结合蛋白，尽管它们的分子量差异很大。在本研究中，我们发现尿白蛋白与大小不同的尿蛋白之间均有关联。有必要开展进一步研究以确定各种肾脏疾病中的细胞因子模式和潜在的病情进展标志物。
