• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

补偿性碱基变化揭示了斯里兰卡广义（双翅目：蚊科）物种复合体成员之间的性不相容性。

Compensatory Base Changes Reveal Sexual Incompatibility among Members of the Sensu Lato (Diptera: Culicidae) Species Complex in Sri Lanka.

作者信息

Jayatunga D P W, Harischandra I N, Chandrasekharan N V, de Silva B G D N K

机构信息

Center for Biotechnology, Department of Zoology, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka.

Genetics and Molecular Biology Unit, Faculty of Applied Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka.

出版信息

Life (Basel). 2021 Mar 8;11(3):211. doi: 10.3390/life11030211.

DOI:10.3390/life11030211
PMID:33800295
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7998985/
Abstract

The mosquito sensu lato (s.l.) is a major secondary vector of malaria in Sri Lanka. The sibling species composition in this species complex in Sri Lanka remains debatable. Compensatory base changes (CBCs) in the secondary structures of internal transcribed spacer 2 (ITS2) are reliable sources to predict sexual incompatibility among closely related species. The objective of the present study was to investigate the s.l. populations in Sri Lanka using the CBC analysis. Mosquito DNA was amplified and sequenced for the ITS2 region. The sequences were annotated using ITS2 Database. ITS2 secondary structures were constructed and analyzed for CBCs using various bioinformatics tools. The ITS2 regions consisted of two different lengths, 575 bp and 480 bp. The two CBCs and three hemi CBCs identified in the present study suggest that there may be at least two sexually incompatible sibling species. In conclusion, it is likely that there may be only two reproductively isolated sibling species in the species complex in Sri Lanka. However, due to high divergence of ITS2 in these species, it is reasonable to assume that they may be undergoing a speciation event to separate as a distinct species.

摘要

广义的蚊子是斯里兰卡疟疾的主要次要传播媒介。斯里兰卡该蚊种复合体中的同胞物种组成仍存在争议。内转录间隔区2（ITS2）二级结构中的补偿性碱基变化（CBCs）是预测近缘物种间性不相容性的可靠来源。本研究的目的是利用CBC分析调查斯里兰卡的广义蚊子种群。对蚊子DNA的ITS2区域进行扩增和测序。使用ITS2数据库对序列进行注释。构建ITS2二级结构，并使用各种生物信息学工具分析CBCs。ITS2区域由两种不同长度组成，575 bp和480 bp。本研究中鉴定出的两个CBCs和三个半CBCs表明可能至少有两个性不相容的同胞物种。总之，斯里兰卡该蚊种复合体中可能只有两个生殖隔离的同胞物种。然而，由于这些物种中ITS2的高度分化，有理由假设它们可能正在经历一个物种形成事件以分化为不同的物种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/5e51ecb9cbfc/life-11-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/523bd08e6866/life-11-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/a2b41da68809/life-11-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/84bd0db3f3d3/life-11-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/871bccc78364/life-11-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/5e51ecb9cbfc/life-11-00211-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/523bd08e6866/life-11-00211-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/a2b41da68809/life-11-00211-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/84bd0db3f3d3/life-11-00211-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/871bccc78364/life-11-00211-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ae3/7998985/5e51ecb9cbfc/life-11-00211-g005.jpg

相似文献

1
Compensatory Base Changes Reveal Sexual Incompatibility among Members of the Sensu Lato (Diptera: Culicidae) Species Complex in Sri Lanka.补偿性碱基变化揭示了斯里兰卡广义（双翅目：蚊科）物种复合体成员之间的性不相容性。
Life (Basel). 2021 Mar 8;11(3):211. doi: 10.3390/life11030211.
2
Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka.来自斯里兰卡八个地理位置的按蚊（双翅目：蚊科）蚊子的分子特征分析
Malar J. 2017 Jun 2;16(1):234. doi: 10.1186/s12936-017-1876-y.
3
Molecular characterization and identification of members of the Anopheles subpictus complex in Sri Lanka.斯里兰卡塞蚊亚属复合体成员的分子特征描述与鉴定。
Malar J. 2013 Aug 30;12:304. doi: 10.1186/1475-2875-12-304.
4
Genetic evidence for malaria vectors of the Anopheles sundaicus complex in Sri Lanka with morphological characteristics attributed to Anopheles subpictus species B.斯里兰卡具有形态特征归因于亚种 B 的按蚊复合体致疟媒介的遗传证据
Malar J. 2010 Nov 29;9:343. doi: 10.1186/1475-2875-9-343.
5
Molecular forms of Anopheles subpictus and Anopheles sundaicus in the Indian subcontinent.印度次大陆的暗蚊属和煞蚊属的分子形态。
Malar J. 2020 Nov 19;19(1):417. doi: 10.1186/s12936-020-03492-2.
6
Alterations and Interchange of Morphometric Characters in Different Life Cycle Stages with Reference to Genomic Variations of (Diptera; Culicidae) Sibling Species Complex in Sri Lanka.参考斯里兰卡（双翅目；蚊科）姐妹种复合体的基因组变异，探讨不同生命周期阶段形态特征的改变与互换
Insects. 2018 Jul 24;9(3):89. doi: 10.3390/insects9030089.
7
Genetic diversity and population structure of malaria vector mosquitoes Anopheles subpictus, Anopheles peditaeniatus, and Anopheles vagus in five districts of Sri Lanka.斯里兰卡五个地区的疟疾病媒蚊按蚊 subpictus、按蚊 peditaeniatus 和按蚊 vagus 的遗传多样性和种群结构。
Malar J. 2018 Jul 20;17(1):271. doi: 10.1186/s12936-018-2419-x.
8
Variations in salinity tolerance of malaria vectors of the Anopheles subpictus complex in Sri Lanka and the implications for malaria transmission.斯里兰卡寡鬃实蝇复合体疟蚊耐盐性的变化及其对疟疾传播的影响。
Parasit Vectors. 2011 Jun 24;4:117. doi: 10.1186/1756-3305-4-117.
9
Bionomic aspects of the Anopheles subpictus species complex in Sri Lanka.斯里兰卡拟暗按蚊种群复合体的生物学特性
J Insect Sci. 2014;14:97. doi: 10.1673/031.014.97.
10
Integrated systematics of Anopheles subpictus (Diptera: Culicidae) in the Oriental Region, with emphasis on forms in Thailand and Sulawesi, Indonesia.东方地区拟暗按蚊（双翅目：蚊科）的综合分类学，重点关注泰国和印度尼西亚苏拉威西岛的形态。
Acta Trop. 2020 Aug;208:105503. doi: 10.1016/j.actatropica.2020.105503. Epub 2020 May 11.

引用本文的文献

1
Genetic diversity, phylogenetic and phylogeographic analysis of Anopheles culicifacies species complex using ITS2 and COI sequences.应用 ITS2 和 COI 序列对库蚊复合种的遗传多样性、系统发育和系统地理学分析。
PLoS One. 2023 Aug 16;18(8):e0290178. doi: 10.1371/journal.pone.0290178. eCollection 2023.

本文引用的文献

1
Molecular forms of Anopheles subpictus and Anopheles sundaicus in the Indian subcontinent.印度次大陆的暗蚊属和煞蚊属的分子形态。
Malar J. 2020 Nov 19;19(1):417. doi: 10.1186/s12936-020-03492-2.
2
Integrated systematics of Anopheles subpictus (Diptera: Culicidae) in the Oriental Region, with emphasis on forms in Thailand and Sulawesi, Indonesia.东方地区拟暗按蚊（双翅目：蚊科）的综合分类学，重点关注泰国和印度尼西亚苏拉威西岛的形态。
Acta Trop. 2020 Aug;208:105503. doi: 10.1016/j.actatropica.2020.105503. Epub 2020 May 11.
3
Cryptic species in the parasitic Amoebophrya species complex revealed by a polyphasic approach.
多相研究揭示寄生性变形虫物种复合体中的隐秘物种。
Sci Rep. 2020 Feb 13;10(1):2531. doi: 10.1038/s41598-020-59524-z.
4
Alternative analyses of compensatory base changes in an ITS2 phylogeny of Corydalis (Papaveraceae).ITS2 系统发育中对紫堇属（罂粟科）补偿碱基变化的替代分析。
Ann Bot. 2019 Sep 24;124(2):233-243. doi: 10.1093/aob/mcz062.
5
Alterations and Interchange of Morphometric Characters in Different Life Cycle Stages with Reference to Genomic Variations of (Diptera; Culicidae) Sibling Species Complex in Sri Lanka.参考斯里兰卡（双翅目；蚊科）姐妹种复合体的基因组变异，探讨不同生命周期阶段形态特征的改变与互换
Insects. 2018 Jul 24;9(3):89. doi: 10.3390/insects9030089.
6
MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms.MEGA X：跨越计算平台的分子进化遗传学分析。
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549. doi: 10.1093/molbev/msy096.
7
Molecular characterization of Anopheline (Diptera: Culicidae) mosquitoes from eight geographical locations of Sri Lanka.来自斯里兰卡八个地理位置的按蚊（双翅目：蚊科）蚊子的分子特征分析
Malar J. 2017 Jun 2;16(1):234. doi: 10.1186/s12936-017-1876-y.
8
Bionomic aspects of the Anopheles subpictus species complex in Sri Lanka.斯里兰卡拟暗按蚊种群复合体的生物学特性
J Insect Sci. 2014;14:97. doi: 10.1673/031.014.97.
9
Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli (family Hesperiidae) comprises three morphologically cryptic species.线粒体和核系统发育分析，包括 Sanger 和新一代测序技术，表明在哥斯达黎加西北部的瓜纳卡斯特保护区，一种名为 Urbanus belli（鳞翅目：弄蝶科）的弄蝶包含 3 个形态上隐秘的物种。
BMC Evol Biol. 2014 Jul 9;14:153. doi: 10.1186/1471-2148-14-153.
10
Adaptive introgression between Anopheles sibling species eliminates a major genomic island but not reproductive isolation.按蚊近缘种之间的适应性基因渐渗消除了一个主要的基因组岛，但并未消除生殖隔离。
Nat Commun. 2014 Jun 25;5:4248. doi: 10.1038/ncomms5248.