Kunnaja Phraepakaporn, Chansakaow Sunee, Wittayapraparat Absorn, Yusuk Pedcharada, Sireeratawong Seewaboon
Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Molecules. 2021 Mar 28;26(7):1906. doi: 10.3390/molecules26071906.
In Thailand, people in the highland communities whose occupational exposure to pesticides used the root of as a detoxifying agent. However, the scientific data to support the traditional use of this plant are insufficient. This study aimed to evaluate the antioxidant activity and anti-pesticide potential of root extract. Antioxidant properties were investigated by 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay, superoxide radicals scavenging assay, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay, ferric reducing antioxidant power (FRAP), and total phenolic content determination. In all assays, extracts and their fractions exhibited high antioxidant activities differently. The water extract is traditionally used as a detoxifying agent. Therefore, it was chosen for in vivo experiments. The rats received the extract in a way that mimics the traditional methods of tribal communities followed by chlorpyrifos for 16 days. The results showed that acetylcholinesterase activity decreases in pesticide-exposed rats. Treatment with the extract caused increasing acetylcholinesterase activity in the rats. Therefore, extract may potentially be used as a detoxifying agent, especially for the chlorpyrifos pesticide. The antioxidant properties of may provide a beneficial effect by protecting liver cells from damage caused by free radicals. Histopathology results revealed no liver cell necrosis and showed the regeneration of liver cells in the treatment group. extract did not cause changes in behavior, liver weight, hematological and biochemical profiles of the rats.
在泰国，高地社区的人们在职业接触农药时会使用[植物名称]的根作为解毒剂。然而，支持这种植物传统用途的科学数据并不充分。本研究旨在评估[植物名称]根提取物的抗氧化活性和抗农药潜力。通过2,2'-二苯基-1-苦基肼基（DPPH）法、超氧阴离子自由基清除法、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）法、铁还原抗氧化能力（FRAP）以及总酚含量测定来研究抗氧化性能。在所有实验中，[植物名称]提取物及其馏分均表现出不同程度的高抗氧化活性。水提取物在传统上被用作解毒剂。因此，它被选用于体内实验。大鼠以模拟部落社区传统方法的方式接受提取物，随后给予毒死蜱，持续16天。结果表明，暴露于农药的大鼠体内乙酰胆碱酯酶活性降低。用提取物处理使大鼠体内乙酰胆碱酯酶活性增加。因此，[植物名称]提取物可能有潜力用作解毒剂，尤其是针对毒死蜱农药。[植物名称]的抗氧化特性可能通过保护肝细胞免受自由基造成的损伤而产生有益作用。组织病理学结果显示治疗组没有肝细胞坏死，并且显示出肝细胞的再生。[植物名称]提取物没有引起大鼠行为、肝脏重量、血液学和生化指标的变化。
