Taychaworaditsakul Weerakit, Sawong Suphunwadee, Intatham Supaporn, Chansakaow Sunee, Kunnaja Phraepakaporn, Chewonarin Teera, Jaijoy Kanjana, Wittayapraparat Absorn, Yusuk Pedcharada, Sireeratawong Seewaboon
Clinical Research Center for Food and Herbal Product Trials and Development (CR-FAH), Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Pharmacology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand.
Foods. 2024 May 28;13(11):1695. doi: 10.3390/foods13111695.
root's antioxidant and acetylcholinesterase (AChE) activity showed promise as a pesticide detoxification agent in our previous study. In addition to its root, leaves can help alleviate pesticide exposure, although there is limited scientific evidence supporting their efficacy. However, the use of roots in several countries, such as Thailand, could contribute to environmental degradation, as highland communities traditionally used leaves instead of roots. This study aims to evaluate the antioxidant activity and anti-pesticide potential of water extract from leaves through in vitro and in vivo investigations. In the in vitro study, water extract and its fractions demonstrated antioxidant activity and induced apoptosis in hepatic satellite cells. In the in vivo study, treatment with the leaf extract led to increased AChE activity, decreased malondialdehyde (MDA) levels, increased superoxide dismutase (SOD) levels, and reduced glutathione in chlorpyrifos-exposed rats. Histopathological examination revealed that chlorpyrifos-treated rats exhibited liver cell damage, while treatment with the water extract of exhibited a protective effect on the liver. In conclusion, water extract exhibited antioxidant activity, enhanced AChE activity, and improved histopathological abnormalities in the liver.
在我们之前的研究中，[植物名称]根的抗氧化和乙酰胆碱酯酶（AChE）活性显示出作为农药解毒剂的潜力。除了根之外，其叶也有助于减轻农药暴露，尽管支持其功效的科学证据有限。然而，在泰国等几个国家使用根可能会导致环境退化，因为高地社区传统上使用叶而不是根。本研究旨在通过体外和体内研究评估叶水提取物的抗氧化活性和抗农药潜力。在体外研究中，水提取物及其馏分表现出抗氧化活性并诱导肝卫星细胞凋亡。在体内研究中，用叶提取物处理导致毒死蜱暴露大鼠的AChE活性增加、丙二醛（MDA）水平降低、超氧化物歧化酶（SOD）水平升高以及谷胱甘肽减少。组织病理学检查显示，毒死蜱处理的大鼠表现出肝细胞损伤，而用[植物名称]水提取物处理对肝脏具有保护作用。总之，[植物名称]水提取物表现出抗氧化活性，增强了AChE活性，并改善了肝脏的组织病理学异常。
