Cui Xuejing, Gu Xuezhu, Kang Wenyi
Institute of Chinese Materia Medica, Henan University, Kaifeng 475004, China.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing, 100700, P. R. China.
Afr J Tradit Complement Altern Med. 2016 Aug 12;13(5):114-122. doi: 10.21010/ajtcam.v13i5.15. eCollection 2016.
Chinese medicine has its own uniqueness, advantageous in the treatment of hepatic diseases, and they were widely used in the oxidation. At the same time, oxidation is one of the mechanism of protect liver.
In the present study, the antioxidant activity of different extracts from Compound Lobelia were estimated respectively by the methods of measuring the [2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulphonic acid] diamonium salt (ABTS) radical scavenging, ferric reducing antioxidant power (FRAP). The protective effects on carbon tetrachloride (CCl4)-induced acute liver injury in mice which was investigated by analyzing the result of biochemical parameters such as glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in serum, superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue homogenate.
the result showed that ABTS free radical scavenging activity of ethanol extract (IC=29.26±0.49 μg/mL) was stronger than that of water extract (IC50=42.09±2.44 μg/mL), but they were lower than that of BHT as positive control (IC50=2.47±0.09 μg/mL). The ferric reducing antioxidant power of ethanol extract (FRAP=329.03±46.30 μmol/g) was higher than that of water extract (FRAP=206.03±54.30 μmol/g); they were lower than that of BHT (FRAP=1541.87±9.70 μmol/g). Water extract and ethanol extract could significantly reduce GOT activity (<0.001) and GPT activity (<0.001) in serum, reduce significantly the content of MDA (<0.001) and significantly increase SOD activity (<0.001) in the mice liver tissue homogenate.
Compound lobelia had a certain antioxidant and satisfied hepatoprotective effects. Moreover, the liver-protective effect was concerned with antioxidant activity and enhances the body immunity. Compound lobelia is expected to be a new medicine for protecting liver.
中医药具有自身独特性，在肝病治疗方面具有优势，且在氧化方面应用广泛。同时，氧化是肝脏保护机制之一。
在本研究中，分别采用测定2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)二铵盐自由基清除能力、铁还原抗氧化能力(FRAP)的方法，对复方半边莲不同提取物的抗氧化活性进行评估。通过分析血清中谷丙转氨酶(GOT)和谷草转氨酶(GPT)、肝组织匀浆中超氧化物歧化酶(SOD)和丙二醛(MDA)等生化参数的结果，研究其对四氯化碳(CCl4)诱导的小鼠急性肝损伤的保护作用。
结果显示，乙醇提取物的ABTS自由基清除活性(IC50=29.26±0.49 μg/mL)强于水提取物(IC50=42.09±2.44 μg/mL)，但均低于作为阳性对照的BHT(IC50=2.47±0.09 μg/mL)。乙醇提取物的铁还原抗氧化能力(FRAP=329.03±46.30 μmol/g)高于水提取物(FRAP=206.03±54.30 μmol/g)；均低于BHT(FRAP=1541.87±9.70 μmol/g)。水提取物和乙醇提取物均可显著降低血清中GOT活性(<0.001)和GPT活性(<0.001)，显著降低小鼠肝组织匀浆中MDA含量(<0.001)并显著提高SOD活性(<0.001)。
复方半边莲具有一定的抗氧化和良好的肝脏保护作用。此外，肝脏保护作用与抗氧化活性有关，并可增强机体免疫力。复方半边莲有望成为一种新型保肝药物。
