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中国马眼部疾病的首次分子确认

First Molecular Confirmation of Equine Ocular in China.

作者信息

Yu Feng, Liu Bo, Chen Shulei, Yi Ziwen, Liu Xianyong, Zhu Yiping, Li Jing

机构信息

Equine Clinical Diagnostic Center, College of Veterinary Medicine, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100093, China.

Department of Veterinary Parasitology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.

出版信息

Vet Sci. 2021 Mar 28;8(4):55. doi: 10.3390/vetsci8040055.

Abstract

A 5-year-old Mongolian mare ( Linnaeus, 1758) was observed to have corneal opacity and excessive ocular discharge. An ophthalmic examination revealed a moving thread-like cylindrical worm in the anterior chamber of the right eye. The parasite was successfully removed surgically. The worm was observed under light microscopy and confirmed as by 12S rRNA gene amplification and sequencing. Phylogenetic analysis demonstrated similarity with in the National Center for Biotechnology Information (NCBI) GenBank database isolated from other Asian countries. This report is the first confirmed case of equine ocular setariasis by molecular diagnosis in China, which may indicate its presence in livestock and promote research on its epidemiology.

摘要

一匹5岁的蒙古母马(林奈,1758年)被观察到有角膜混浊和眼部分泌物过多的症状。眼科检查发现右眼前房有一条蠕动的线状圆柱形蠕虫。该寄生虫通过手术成功取出。通过光学显微镜观察该蠕虫,并通过12S rRNA基因扩增和测序进行确认。系统发育分析表明,与从其他亚洲国家分离的保藏在美国国立生物技术信息中心(NCBI)基因库数据库中的[具体物种名称未给出]具有相似性。本报告是中国首例通过分子诊断确诊的马眼部丝状虫病病例,这可能表明其在牲畜中的存在,并促进对其流行病学的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/009a/8066632/d2e3a491c575/vetsci-08-00055-g001.jpg

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