来自中国的指形丝状线虫（线虫纲：丝状科）线粒体全基因组的特征分析

Characterization of the complete mitochondrial genome of Setaria digitata (Nematoda: Setariidae) from China.

作者信息

Liu G-H, Li J-Y, Zhu X-Q

机构信息

College of Veterinary Medicine,Hunan Agricultural University,Changsha,Hunan Province 410128,China.

State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Veterinary Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu Province 730046,China.

出版信息

J Helminthol. 2017 Nov;91(6):772-776. doi: 10.1017/S0022149X16000912.

DOI:10.1017/S0022149X16000912

PMID:28100285

Abstract

Setaria digitata is a filarial parasite that causes fatal cerebrospinal nematodiasis in goats, horses and sheep, resulting in substantial economic losses to livestock farmers. In the present study, the complete mitochondrial (mt) genome of S. digitata from China was determined, characterized and compared with that of S. digitata from Sri Lanka. The identity of the mt genomes was 98.3% between S. digitata from China and Sri Lanka, and the complete mt genome sequence of S. digitata from China was slightly shorter (25 bp) than that from Sri Lanka. For the 12 protein genes, this comparison revealed sequence differences at both the nucleotide (1.4%) and amino acid (2.2%) levels. The present study determined the complete mt genome sequence of S. digitata from China, providing novel genetic markers for the study of the population genetics and molecular epidemiology of S. digitata in animals.

摘要

指状丝虫是一种丝状寄生虫，可导致山羊、马和绵羊患上致命的脑脊髓线虫病，给养殖户造成重大经济损失。在本研究中，测定并表征了来自中国的指状丝虫的完整线粒体（mt）基因组，并将其与来自斯里兰卡的指状丝虫的线粒体基因组进行了比较。来自中国和斯里兰卡的指状丝虫的线粒体基因组的同一性为98.3%，来自中国的指状丝虫的完整线粒体基因组序列比来自斯里兰卡的略短（25bp）。对于12个蛋白质基因，这种比较揭示了核苷酸（1.4%）和氨基酸（2.2%）水平上的序列差异。本研究测定了来自中国的指状丝虫的完整线粒体基因组序列，为研究动物中该指状丝虫的群体遗传学和分子流行病学提供了新的遗传标记。