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两种骨骼肌肌动蛋白启动子因子的DNA结合位点对在肌肉细胞中的表达很重要。

DNA-binding site for two skeletal actin promoter factors is important for expression in muscle cells.

作者信息

Walsh K, Schimmel P

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Mol Cell Biol. 1988 Apr;8(4):1800-2. doi: 10.1128/mcb.8.4.1800-1802.1988.

DOI:10.1128/mcb.8.4.1800-1802.1988
PMID:3380098
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC363341/
Abstract

Two nuclear factors bind to the same site in the chicken skeletal actin promoter. Mutations in the footprint sequence which eliminate detectable binding decrease expression in transfected skeletal muscle cells by a factor of 25 to 50 and do not elevate the low expression in nonmuscle cells. These results show that the factor-binding site contributes to the activation of expression in muscle cells and that it alone does not contribute significantly to repress expression in nonmuscle cells.

摘要

两种核因子与鸡骨骼肌肌动蛋白启动子中的同一位点结合。足迹序列中的突变消除了可检测到的结合，使转染的骨骼肌细胞中的表达降低了25至50倍，并且不会提高非肌肉细胞中的低表达水平。这些结果表明，因子结合位点有助于肌肉细胞中表达的激活，并且它本身对非肌肉细胞中表达的抑制作用不显著。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/5d7739f6f860/molcellb00064-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/8ef21e28803e/molcellb00064-0428-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/a7d312255263/molcellb00064-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/5d7739f6f860/molcellb00064-0430-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/8ef21e28803e/molcellb00064-0428-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/a7d312255263/molcellb00064-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3f6/363341/5d7739f6f860/molcellb00064-0430-a.jpg

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本文引用的文献

1
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2
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
3
A protein binds to a satellite DNA repeat at three specific sites that would be brought into mutual proximity by DNA folding in the nucleosome.
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Mol Cell Biol. 1993 May;13(5):2753-64. doi: 10.1128/mcb.13.5.2753-2764.1993.
4
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5
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Mol Cell Biol. 1993 Nov;13(11):6907-18. doi: 10.1128/mcb.13.11.6907-6918.1993.
6
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Nucleic Acids Res. 1989 Jan 25;17(2):601-15. doi: 10.1093/nar/17.2.601.
7
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Mol Cell Biol. 1989 Sep;9(9):3785-92. doi: 10.1128/mcb.9.9.3785-3792.1989.
8
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9
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4
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5
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Dev Biol. 1972 Jun;28(2):407-29. doi: 10.1016/0012-1606(72)90023-1.
7
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Mol Cell Biol. 1986 Jul;6(7):2462-75. doi: 10.1128/mcb.6.7.2462-2475.1986.
8
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Mol Cell Biol. 1986 Jun;6(6):2125-36. doi: 10.1128/mcb.6.6.2125-2136.1986.
9
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J Biol Chem. 1987 Jul 15;262(20):9429-32.
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Mol Cell Biol. 1986 Sep;6(9):3173-9. doi: 10.1128/mcb.6.9.3173-3179.1986.