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[H3-胸腺嘧啶核苷掺入处于减数分裂前期I的小体鲟鱼卵母细胞中的情况]

[Incorporation of H3-thymidine into the oocytes of a sterlet in the early meiotic prophase].

作者信息

Chmilevskiĭ D A, Raĭkova E V

出版信息

Tsitologiia. 1976;18(6):689-92.

PMID:1027159
Abstract

DNA synthesis in meiotic oocytes of the sterlet (A. ruthenus) has been studied during early prophase stages using H3-thymidine. The pattern of H3-thymidine incorporation is similar to that in oocytes of Amphibia and Osteichthyes. In the oogonia as well as in the leptotene and zygotene oocytes, the label is predominantly localized over chromosomes. An intensive incorporation of H3-thymidine into the material of the heterochromatic "cap" has been observed during pachytene. Thus, the main synthesis of extra DNA in the sterlet oocytes occurs during pachytene. No DNA in synthesized by the diplotene oocytes.

摘要

利用H3-胸腺嘧啶核苷对小体鲟(A. ruthenus)减数分裂期卵母细胞早期前期阶段的DNA合成进行了研究。H3-胸腺嘧啶核苷的掺入模式与两栖类和硬骨鱼类卵母细胞中的相似。在卵原细胞以及细线期和偶线期卵母细胞中,标记主要定位于染色体上。在粗线期观察到H3-胸腺嘧啶核苷大量掺入异染色质“帽”的物质中。因此,小体鲟卵母细胞中额外DNA的主要合成发生在粗线期。双线期卵母细胞不合成DNA。

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