Aras Sadiye, Kabir Niamul, Wadood Sabrina, George Jyothi, Chowdhury Shahid, Fouladkhah Aliyar Cyrus
Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA.
Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA.
Microorganisms. 2021 Mar 21;9(3):653. doi: 10.3390/microorganisms9030653.
The inactivation of bacterial endospores continues to be the main curtailment for further adoption of high-pressure processing in intrastate, interstate, and global food commerce. The current study investigated the effects of elevated hydrostatic pressure for the inactivation of endospore suspension of three indicator spore-forming bacteria of concern to the food industry. Additionally, the effects of four bacteriocin/bactericidal compounds were studied for augmenting the decontamination efficacy of the treatment. Elevated hydrostatic pressure at 650 MPa and at 50 °C was applied for 0 min (untreated control) and for 3, 7, and 11 min with and without 50K IU of nisin, 224 mg/L lysozyme, 1% lactic acid, and 1% Citricidal. The results were statistically analyzed using Tukey- and Dunnett's-adjusted ANOVA. Under the condition of our experiments, we observed that a well-designed pressure treatment synergized with mild heat and bacteriocin/bactericidal compounds could reduce up to >4 logs CFU/mL (i.e., >99.99%) of bacterial endospores. Additions of nisin and lysozyme were able, to a great extent, to augment ( < 0.05) the decontamination efficacy of pressure-based treatments against and , while exhibiting no added benefit ( ≥ 0.05) for reducing endospores of . The addition of lactic acid, however, was efficacious for augmenting the pressure-based reduction of bacterial endospores of the three microorganisms.
细菌芽孢的失活仍然是高压处理在州内、州际和全球食品商业中进一步应用的主要限制因素。本研究调查了升高的静水压力对食品工业关注的三种指示性产芽孢细菌芽孢悬液失活的影响。此外，还研究了四种细菌素/杀菌化合物对增强处理去污效果的影响。在650兆帕和50℃的升高静水压力下，分别在0分钟（未处理对照）、3分钟、7分钟和11分钟施加压力，施加压力时分别添加和不添加50000国际单位的乳酸链球菌素、224毫克/升溶菌酶、1%乳酸和1%柠檬酸。使用Tukey和Dunnett调整的方差分析对结果进行统计分析。在我们的实验条件下，我们观察到精心设计的压力处理与温和加热以及细菌素/杀菌化合物协同作用，可以减少高达>4个对数CFU/毫升（即>99.99%）的细菌芽孢。添加乳酸链球菌素和溶菌酶在很大程度上能够增强（<0.05）基于压力的处理对 和 的去污效果，而对于减少 的芽孢没有额外益处（≥0.05）。然而，添加乳酸对于增强基于压力的三种微生物细菌芽孢的减少是有效的。
