Public Health Microbiology Laboratory, Tennessee State University, Nashville, TN 37209, USA.
Department of Biological Sciences, Tennessee State University, Nashville, TN 37209, USA.
Int J Environ Res Public Health. 2020 Sep 25;17(19):7033. doi: 10.3390/ijerph17197033.
Current study investigated effects of elevated hydrostatic pressure exposure in the presence of mild heat and natural antimicrobials against . Hydrostatic pressure of 350 to 550 MPa with nisin (5000 IU/mL), carvacrol, or caprylic acid (0.5% /) were applied for the reduction in four-strain mixture of in HEPES buffer at 4 and 40 °C for up to 7 min. Results were statistically analyzed by ANOVA and D-values were additionally calculated using best-fitted linear model. Prior to exposure to treatments at 4 °C, counts of the pathogen were 7.95 ± 0.4 log CFU/mL and were reduced ( < 0.05) to 6.44 ± 0.3 log CFU/mL after 7 min of treatment at 450 MPa. D-value associated with this treatment was 5.34 min (R = 0.72). At 40 °C, counts were 8.21 ± 0.7 and 5.77 ± 0.3 log CFU/mL before and after the 7-min treatments, respectively. D-value associated with 40 °C treatment was 3.30 min (R = 0.62). Application of the antimicrobials provided additional pathogen reduction augmentation for treatments < 5 min. The results of the current study could be incorporated for meeting regulatory requirements such as Food Code, HACCP, and Preventive Control for Human Food of Food Safety Modernization Act for assuring microbiological safety of products against this prevalent pathogen of public health concern.
当前研究调查了在温和热和天然抗菌剂存在的情况下升高静水压力对 的影响。在 4 和 40°C 下，将 350 至 550 MPa 的静水压力与乳链菌肽（5000 IU/mL）、香芹酚或辛酸（0.5%/）一起应用于 HEPEs 缓冲液中的四株混合菌，持续 7 分钟，以减少 的数量。结果通过方差分析进行了统计学分析，并使用最佳拟合线性模型计算了 D 值。在 4°C 下进行处理之前，病原体的计数为 7.95 ± 0.4 log CFU/mL，在 450 MPa 下处理 7 分钟后减少（<0.05）至 6.44 ± 0.3 log CFU/mL。与该处理相关的 D 值为 5.34 分钟（R = 0.72）。在 40°C 下，处理前和处理后分别为 8.21 ± 0.7 和 5.77 ± 0.3 log CFU/mL。与 40°C 处理相关的 D 值为 3.30 分钟（R = 0.62）。抗菌剂的应用为处理时间<5 分钟提供了额外的病原体减少增强效果。本研究的结果可用于满足法规要求，例如食品安全现代化法案的食品法典、HACCP 和人类食品的预防性控制，以确保产品对公众健康关注的这种普遍病原体的微生物安全性。
