不同平均寿命的秀丽隐杆线虫品系的辐射敏感性和DNA修复

Radiation sensitivity and DNA repair in Caenorhabditis elegans strains with different mean life spans.

作者信息

Hartman P S, Simpson V J, Johnson T, Mitchell D

机构信息

Department of Biology, Texas Christian University, Fort Worth 76129.

出版信息

Mutat Res. 1988 Jun;208(2):77-82. doi: 10.1016/s0165-7992(98)90003-3.

DOI:10.1016/s0165-7992(98)90003-3

PMID:3380112

Abstract

The sensitivities to three DNA damaging agents (UV and gamma-radiation, methyl methanesulfonate) were measured in four recombinant inbred (RI) strains of Caenorhabditis elegans with mean life spans ranging from 13 to 30.9 days, as well as in the wild-type strains used to derive these RI's. Sensitivities at several stages in the developmental cycle were tested. There were no significant correlations between mean life span and the lethal effects of these 3 agents. Excision of two UV-radiation-induced DNA photoproducts was also measured. Long-lived strains were no more repair competent than shorter-lived strains. These data indicate that DNA repair plays at best a minor role in the aging process of C. elegans.

摘要

在平均寿命从13天到30.9天不等的四种秀丽隐杆线虫重组近交（RI）品系中，以及在用于培育这些RI品系的野生型品系中，测定了对三种DNA损伤剂（紫外线和γ辐射、甲基磺酸甲酯）的敏感性。测试了发育周期中几个阶段的敏感性。平均寿命与这三种试剂的致死效应之间没有显著相关性。还测定了两种紫外线诱导的DNA光产物的切除情况。长寿品系的修复能力并不比短寿品系更强。这些数据表明，DNA修复在秀丽隐杆线虫的衰老过程中充其量只起次要作用。