秀丽隐杆线虫寿命的遗传分析。
Genetic analysis of life-span in Caenorhabditis elegans.
作者信息
Johnson T E, Wood W B
出版信息
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6603-7. doi: 10.1073/pnas.79.21.6603.
Crosses between Bristol and Bergerac strains of the self-fertilizing hermaphroditic nematode Caenorhabditis elegans do not show the heterosis effects for life-span that complicate analysis of interstrain crosses with Drosophila or mice. Instead they yield F1 progeny with life-spans similar to those of the parent strains. By analysis of life-span variation among progeny F2 populations from such crosses and by two independent analyses of life-spans among recombinant inbred lines derived from F2 individuals by 18 rounds of self-fertilization, we estimate that the heritability of life-span in C. elegans is between 20% and 50%. Recombinant inbred lines show a range in mean life-spans of 10 days to 31 days compared to life-spans of about 18 days for each of the two parental strains. We conclude that life-span variation in C. elegans has a substantial genetic component and that this organism offers promising opportunities for selective breeding of longer-lived strains and genetic analysis of senescence.
自体受精的雌雄同体线虫秀丽隐杆线虫的布里斯托尔品系和贝杰拉克品系之间的杂交，并未表现出影响寿命的杂种优势效应，而这种效应会使果蝇或小鼠品系间杂交的分析变得复杂。相反，它们产生的F1代后代的寿命与亲本品系相似。通过分析此类杂交后代F2群体中的寿命变异，以及对由F2个体经过18轮自体受精产生的重组自交系的寿命进行两次独立分析，我们估计秀丽隐杆线虫寿命的遗传力在20%至50%之间。与两个亲本品系各自约18天的寿命相比，重组自交系的平均寿命范围在10天至31天之间。我们得出结论，秀丽隐杆线虫的寿命变异具有很大的遗传成分，并且这种生物为培育寿命更长的品系进行选择性育种以及衰老的遗传分析提供了广阔的机会。
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