Erper Ismail, Ozer Goksel, Kalendar Ruslan, Avci Sirin, Yildirim Elif, Alkan Mehtap, Turkkan Muharrem
Department of Plant Protection, Faculty of Agriculture, Ondokuz Mayis University, Atakum, 55139 Samsun, Turkey.
Department of Plant Protection, Faculty of Agriculture, Kyrgyz Turkish Manas University, Bishkek 720044, Kyrgyzstan.
J Fungi (Basel). 2021 Mar 21;7(3):234. doi: 10.3390/jof7030234.
A total of 132 isolates were recovered from red cabbage plants with root rot and wirestem symptoms in the province of Samsun (Turkey) between 2018 and 2019. Based on the sequence analysis of the internal transcribed spacer (ITS) region located between the 18S and 28S ribosomal RNA genes and including nuclear staining, these 124 isolates were assigned to multinucleate , and eight were binucleate . The most prevalent anastomosis group (AG) was AG 4 (84%), which was subdivided into AG 4 HG-I (81%) and AG 4 HG-III (3%), followed by AG 5 (10%) and AG-A (6%), respectively. The unweighted pair group method phylogenetic tree resulting from the data of 68 isolates with the inter-PBS amplification DNA profiling method based on interspersed retrotransposon element sequences confirmed the differentiation of AGs with a higher resolution. In the greenhouse experiment with representative isolates ( = 24) from AGs on red cabbage (cv. Rondale), the disease severity index was between 3.33 and 4.0 for multinucleate AG isolates and ranged from 2.5 to 3.17 for AG-A isolates. In the pathogenicity assay of six red cabbage cultivars, one isolate for each AG was tested using a similar method, and all cultivars were susceptible to AG 4 HG-I and AG 4 HG-III isolates. Redriver and Remale were moderately susceptible, while Rescue, Travero, Integro, and Rondale were susceptible to the AG 5 isolate. The results indicate that the most prevalent and aggressive AGs of are devastating pathogens to red cabbage, which means that rotation with nonhost-crops for these AGs may be the most effective control strategy. This is the first comprehensive study isolates in red cabbage using a molecular approach to assess genetic diversity using iPBS-amplified DNA profiling.
2018年至2019年期间，在土耳其萨姆松省，从患有根腐病和猝倒症状的红甘蓝植株中总共分离出132个菌株。基于对位于18S和28S核糖体RNA基因之间且包含核染色的内转录间隔区（ITS）区域的序列分析，这124个菌株被归类为多核的，8个为双核的。最普遍的融合群（AG）是AG 4（84%），它又细分为AG 4 HG-I（81%）和AG 4 HG-III（3%），其次分别是AG 5（10%）和AG-A（6%）。基于散布重复反转录转座子元件序列的PBS间扩增DNA谱分析方法，对68个菌株的数据构建的非加权配对组方法系统发育树，以更高的分辨率证实了融合群的分化。在对红甘蓝（品种Rondale）上来自不同融合群的代表性菌株（n = 24）进行的温室试验中，多核AG分离株的病情严重指数在3.33至4.0之间，AG-A分离株的病情严重指数在2.5至3.17之间。在对六个红甘蓝品种的致病性测定中，使用类似方法对每个融合群的一个分离株进行了测试, 所有品种对AG 4 HG-I和AG 4 HG-III分离株均易感。Redriver和Remale中度易感，而Rescue、Travero、Integro和Rondale对AG 5分离株易感。结果表明，最普遍且具有侵袭性的融合群是红甘蓝的毁灭性病原菌，这意味着与这些融合群的非寄主作物轮作可能是最有效的控制策略。这是首次使用基于iPBS扩增DNA谱分析的分子方法对红甘蓝中的分离株进行全面研究以评估遗传多样性。
