Kalendar Ruslan, Boronnikova Svetlana, Seppänen Mervi
Department of Agricultural Sciences, Viikki Plant Science Centre and Helsinki Sustainability Centre, University of Helsinki, Helsinki, Finland.
National Laboratory Astana, Nazarbayev University, Nur-Sultan, Kazakhstan.
Methods Mol Biol. 2021;2222:57-67. doi: 10.1007/978-1-0716-0997-2_3.
The isolation of nucleic acids from a biological sample is an important step for many molecular biology applications and medical diagnostic assays. This chapter describes an efficient protocol using established acidic CTAB (with a pH value of 5.0 to 6.8) based extraction method for isolation and/or purification of high molecular weight genomic DNA from a range of fresh and difficult sources from plant, animal, fungi, and soil material. This protocol is suitable for many sequencing and genotyping applications, including large-scale sample screening.
从生物样品中分离核酸是许多分子生物学应用和医学诊断检测的重要步骤。本章介绍了一种高效的方法，该方法采用既定的酸性十六烷基三甲基溴化铵（pH值为5.0至6.8）提取法，用于从植物、动物、真菌和土壤材料等一系列新鲜且难以处理的来源中分离和/或纯化高分子量基因组DNA。该方法适用于许多测序和基因分型应用，包括大规模样品筛选。
