First Department of Obstetrics and Gynaecology, Centre of Postgraduate Medical Education, Żelazna 90, 01-004 Warsaw, Poland.
Department of Medical Biology and Genetics, Faculty of Biology University of Gdańsk, Wita Stwosza 59, 80-308 Gdańsk, Poland.
Int J Mol Sci. 2021 Mar 21;22(6):3195. doi: 10.3390/ijms22063195.
It was suggested that the epigenetic alterations of the placenta are associated with obesity, as well as the delivery mode. This study aimed to assess the effect of maternal outcome and delivery procedure on global placental DNA methylation status, as well as selected 5'-Cytosine-phosphate-Guanine-3' (CpG) sites in and genes. Global DNA methylation profile in the placenta was assessed using the 5-methylcytosine (5-mC) and 5-hydroxymethylcytosine (5-hmC) ratio evaluated with the ELISA, followed by target gene methylation patterns at selected gene regions which were determined using methylation-specific qPCR in 70 placentas from healthy, pregnant women with single pregnancy. We found no statistically significant differences in 5-mC/5-hmC ratio between intrapartum cesarean sections (CS) and vaginal deliveries ( = 0.214), as well as between elective cesarean sections and vaginal deliveries ( = 0.221). In intrapartum cesarean sections, the demethylation index was significantly higher (the average: 1.75) compared to elective cesarean section (the average: 1.23, = 0.010) and vaginal deliveries (the average: 1.23, = 0.011). The demethylation index did not significantly differ among elective CS, intrapartum CS, and vaginal delivery groups. The demethylation index of correlated negatively with in the placenta in the vaginal delivery group ( = -0.456, = 0.017), but not with the global methylation. The methylation of a singular locus might be different depending on the mode of delivery and uterine contractions. Further studies should be conducted with locus-specific analysis of the whole genome to detect the methylation index of specific genes involved in metabolism.
有人提出，胎盘的表观遗传改变与肥胖以及分娩方式有关。本研究旨在评估母婴结局和分娩方式对胎盘整体 DNA 甲基化状态以及 和 基因中选定的 5'-胞嘧啶-磷酸-鸟嘌呤-3'（CpG）位点的影响。使用 ELISA 评估胎盘 5-甲基胞嘧啶（5-mC）和 5-羟甲基胞嘧啶（5-hmC）的比例来评估整体 DNA 甲基化谱，然后使用甲基化特异性 qPCR 确定选定基因区域的选定基因的甲基化模式在 70 个来自健康、单胎妊娠的孕妇的胎盘。我们发现，阴道分娩和选择性剖宫产之间（ = 0.214）以及阴道分娩和选择性剖宫产之间（ = 0.221）5-mC/5-hmC 比值无统计学差异。在选择性剖宫产中，去甲基化指数明显高于阴道分娩（平均：1.75）（平均：1.23， = 0.010）和选择性剖宫产（平均：1.23， = 0.011）。选择性 CS、择期 CS 和阴道分娩组之间的 去甲基化指数无显着差异。胎盘中 的去甲基化指数与阴道分娩组中的 呈负相关（ = -0.456， = 0.017），但与整体甲基化无关。单一基因座的去甲基化指数可能因分娩方式和子宫收缩而不同。应进行进一步的研究，对整个基因组进行特定基因座的分析，以检测特定基因的甲基化指数参与代谢。
