Kondrotienė Aistė, Daukša Albertas, Pamedytytė Daina, Kazokaitė Mintautė, Žvirblienė Aurelija, Daukšienė Dalia, Simanavičienė Vaida, Klimaitė Raimonda, Golubickaitė Ieva, Stakaitis Rytis, Šarauskas Valdas, Verkauskienė Rasa, Žilaitienė Birutė
Institute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Institute of Digestive Research, Medical Academy, Faculty of Medicine, Lithuanian University of Health Sciences, LT-50161 Kaunas, Lithuania.
Diagnostics (Basel). 2021 Mar 2;11(3):418. doi: 10.3390/diagnostics11030418.
We analyzed miR-146b, miR-21, miR-221, miR-21, and miR-181b in formalin fixed paraffin-embedded papillary thyroid carcinoma (PTC) tissue samples of 312 individuals and evaluated their expression relationship with clinicopathological parameters. A higher expression of miR-21 was related to unifocal lesions ( < 0.011) and autoimmune thyroiditis (0.007). miR-221, miR-222 expression was higher in the PTC tissue samples with extrathyroidal extension ( = 0.049, 0.003, respectively). In a group of PTC patients with pT1a and pT1b sized tumors, the expression of miR-146b, miR-21, miR-221, and miR-222 in PTC tissue samples was lower than in patients with pT2, pT3, and pT4 ( = 0.032; 0.0044; 0.003; 0.001; 0.001, respectively). Patients with lymph node metastases had higher expression of miR-21, -221, -222, and -181b ( < 0.05). A high expression of miR-146b, miR-21, miR-221 panel was associated with decreased overall survival (OS) (Log rank = 0.019). Univariate analysis revealed that presence of metastatic lymph nodes and high expression of miR-146b, miR-21, and miR-221 panels were associated with increased hazard of shorter OS. After multivariate analysis, only sex (male) and age (≥55 years) emerged as independent prognostic factors associated with shorter OS (HR 0.28 (95% CI 0.09-0.86) and HR 0.05 (95% CI 0.01-0.22), respectively). In conclusion, 5 analyzed miRs expression have significant relations to clinicopathologic parameters so further investigations of these molecules are expedient while searching for prognostic PTC biomarkers.
我们分析了312例个体的福尔马林固定石蜡包埋甲状腺乳头状癌（PTC）组织样本中的miR-146b、miR-21、miR-221、miR-21和miR-181b，并评估了它们与临床病理参数的表达关系。miR-21的高表达与单发病变（P<0.011）和自身免疫性甲状腺炎（P = 0.007）相关。miR-221、miR-222在有甲状腺外侵犯的PTC组织样本中表达较高（分别为P = 0.049、0.003）。在一组肿瘤大小为pT1a和pT1b的PTC患者中，PTC组织样本中miR-146b、miR-21、miR-221和miR-222的表达低于pT2、pT3和pT4患者（分别为P = 0.032；0.0044；0.003；0.001；0.001）。有淋巴结转移的患者miR-21、-221、-222和-181b表达较高（P<0.05）。miR-146b、miR-21、miR-221高表达与总生存期（OS）降低相关（对数秩检验P = 0.019）。单因素分析显示，有转移淋巴结以及miR-146b、miR-21和miR-221高表达与较短OS的风险增加相关。多因素分析后，只有性别（男性）和年龄（≥55岁）是与较短OS相关的独立预后因素（风险比分别为0.28（95%可信区间0.09 - 0.86）和0.05（95%可信区间0.01 - 0.22））。总之，所分析的5种miRs表达与临床病理参数有显著关系，因此在寻找PTC预后生物标志物时，对这些分子进行进一步研究是适宜的。
