Levi Michela, Muscatello Luisa Vera, Brunetti Barbara, Benazzi Cinzia, Parenti Federico, Gobbo Francesca, Avallone Giancarlo, Bacci Barbara, Zambon Elisa, Valenti Paola, Sarli Giuseppe
Department of Veterinary Medical Sciences, University of Bologna, Ozzano dell'Emilia, 40064 Bologna, Italy.
Ospedale Veterinario, I Portoni Rossi, Zola Predosa, 40069 Bologna, Italy.
Animals (Basel). 2021 Mar 2;11(3):658. doi: 10.3390/ani11030658.
P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) are major actors in multidrug resistance (MDR) phenomenon in both human and canine mammary carcinomas (CMCs). The aim of this study was to investigate an association between the intrinsic expression of P-gp and BCRP compared to the immunophenotypes and outcome in CMCs. Fifty CMCs were evaluated at immunohistochemistry (IHC) for P-gp, BCRP, Estrogen receptor alpha (ER), Progesterone receptors (PR), Human Epidermal Growth Factor Receptor type 2 (HER2), basal cytokeratins 5/6 (CK5/6), Epidermal Growth Factor Receptor 1 (EGFR), and Ki67 proliferation index. P-gp and BCRP positive cases were, respectively, 52% and 74.5%, with a significantly higher expression of BCRP than P-gp. Five immunophenotypes were defined in 37 out of 50 CMCs: 9 (24.3%) Luminal A, 5 (13.5%) Luminal B, 9 (24.3%) HER2 overexpressing, 9 (24.3%) Triple-negative basal-like, and 5 (13.5%) Triple-negative non-basal-like. In all CMCs at least one marker was expressed. Follow-up data were available for 25 animals. The average cancer-specific survival was 739 ± 444 days. A number of CMCs bear a high expression of P-gp and BCRP but no significant association was found between their expression and the immunophenotypes, Ki67 index, the histological grade, and tumor-related death.
P-糖蛋白（P-gp）和乳腺癌耐药蛋白（BCRP）是人类和犬类乳腺癌（CMC）多药耐药（MDR）现象的主要参与者。本研究的目的是调查P-gp和BCRP的内在表达与CMC免疫表型及预后之间的关联。对50例CMC进行免疫组织化学（IHC）评估，检测P-gp、BCRP、雌激素受体α（ER）、孕激素受体（PR）、人表皮生长因子受体2型（HER2）、基底细胞角蛋白5/6（CK5/6）、表皮生长因子受体1（EGFR）和Ki67增殖指数。P-gp和BCRP阳性病例分别为52%和74.5%，BCRP的表达显著高于P-gp。在50例CMC中的37例中定义了五种免疫表型：9例（24.3%）腔面A型、5例（13.5%）腔面B型、9例（24.3%）HER2过表达型、9例（24.3%）三阴性基底样型和5例（13.5%）三阴性非基底样型。所有CMC中至少表达一种标志物。有25只动物的随访数据可用。平均癌症特异性生存期为739±444天。许多CMC高表达P-gp和BCRP，但未发现它们的表达与免疫表型、Ki67指数、组织学分级和肿瘤相关死亡之间存在显著关联。
