Villasis Elizabeth, Garro Katherine, Rosas-Aguirre Angel, Rodriguez Pamela, Rosado Jason, Gave Anthony, Guzman-Guzman Mitchel, Manrique Paulo, White Michael, Speybroeck Niko, Vinetz Joseph Michael, Torres Katherine, Gamboa Dionicia
Laboratorio de Malaria, Laboratorios de Investigación y Desarrollo, Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, 15102, Lima, Peru.
Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima 15102, Peru.
Pathogens. 2021 Mar 2;10(3):282. doi: 10.3390/pathogens10030282.
The measurement of recent malaria exposure can support malaria control efforts. This study evaluated serological responses to an in-house Merozoite Surface Protein 8 (PvMSP8) expressed in a Baculovirus system as sero-marker of recent exposure to (Pv) in the Peruvian Amazon. In a first evaluation, IgGs against PvMSP8 and PvMSP10 proteins were measured by Luminex in a cohort of 422 Amazonian individuals with known history of Pv exposure (monthly data of infection status by qPCR and/or microscopy over five months). Both serological responses were able to discriminate between exposed and non-exposed individuals in a good manner, with slightly higher performance of anti-PvMSP10 IgGs (area under the curve AUC = 0.78 [95% CI = 0.72-0.83]) than anti-PvMSP8 IgGs (AUC = 0.72 [95% CI = 0.67-0.78]) ( = 0.01). In a second evaluation, the analysis by ELISA of 1251 plasma samples, collected during a population-based cross-sectional survey, confirmed the good performance of anti-PvMSP8 IgGs for discriminating between individuals with Pv infection at the time of survey and/or with antecedent of Pv in the past month (AUC = 0.79 [95% CI = 0.74-0.83]). Anti-PvMSP8 IgG antibodies can be considered as a good biomarker of recent Pv exposure in low-moderate transmission settings of the Peruvian Amazon.
近期疟疾暴露的测量可为疟疾防控工作提供支持。本研究评估了对在杆状病毒系统中表达的内部裂殖子表面蛋白8（PvMSP8）的血清学反应，以此作为秘鲁亚马逊地区近期感染疟原虫（Pv）的血清标志物。在首次评估中，通过Luminex检测了422名有已知Pv暴露史的亚马逊地区个体（通过定量聚合酶链反应和/或显微镜检查获得的五个月内每月感染状况数据）队列中针对PvMSP8和PvMSP10蛋白的IgG。两种血清学反应均能较好地区分暴露个体和未暴露个体，抗PvMSP10 IgG的表现略优于抗PvMSP8 IgG（曲线下面积AUC = 0.78 [95%可信区间CI = 0.72 - 0.83]），抗PvMSP8 IgG的AUC为0.72 [95% CI = 0.67 - 0.78]（P = 0.01）。在第二次评估中，对在基于人群的横断面调查中收集的1251份血浆样本进行酶联免疫吸附测定（ELISA）分析，证实抗PvMSP8 IgG在区分调查时感染Pv的个体和/或过去一个月内有Pv感染史的个体方面表现良好（AUC = 0.79 [95% CI = 0.74 - 0.83]）。在秘鲁亚马逊地区低 - 中度传播环境中，抗PvMSP8 IgG抗体可被视为近期Pv暴露的良好生物标志物。
