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新型抗F1和抗LcrV单克隆抗体的表位分组及其在临床鼠疫检测的简单、快速均相时间分辨荧光免疫分析中的应用

Epitope Binning of Novel Monoclonal Anti F1 and Anti LcrV Antibodies and Their Application in a Simple, Short, HTRF Test for Clinical Plague Detection.

作者信息

Mechaly Adva, Vitner Einat B, Levy Yinon, Gur David, Barlev-Gross Moria, Sittner Assa, Koren Michal, Levy Haim, Mamroud Emanuelle, Fisher Morly

机构信息

The Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona 7410001, Israel.

The Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona 7410001, Israel.

出版信息

Pathogens. 2021 Mar 2;10(3):285. doi: 10.3390/pathogens10030285.

DOI:10.3390/pathogens10030285
PMID:33801490
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8001648/
Abstract

Mouse monoclonal antibodies were raised against plague disease biomarkers: the bacterial capsular protein fraction 1 (F1) and the low-calcium response-LcrV virulence factor (Vag). A novel tandem assay, employing BioLayer Interferometry (BLI), enabled the isolation of antibodies against four different epitopes on Vag. The tandem assay was carried out with hybridoma supernatants, circumventing the need for antibody purification. The BioLayer assay was further adopted for characterization of epitope-repetitive antigens, enabling the discovery of two unique epitopes on F1. The selected antibodies were purified and applied as "oligo-clonal" reagents for the immuno-detection of both biomarkers. The developed Homogenous Time Resolved Fluorescence (HTRF) tests were short (10 min) and simple (no washing steps), allowing for detection of 10 ng/mL F1 and 2.5 ng/mL Vag. The tests were successfully applied for detection of disease biomarkers produced by various strains during growth in blood culture vials.

摘要

制备了针对鼠疫疾病生物标志物的小鼠单克隆抗体

细菌荚膜蛋白组分1（F1）和低钙反应LcrV毒力因子（Vag）。一种采用生物层干涉术（BLI）的新型串联检测方法能够分离出针对Vag上四种不同表位的抗体。该串联检测使用杂交瘤细胞上清液进行，无需抗体纯化。生物层检测进一步用于表位重复抗原的表征，从而发现了F1上的两个独特表位。所选抗体经纯化后用作“寡克隆”试剂，用于两种生物标志物的免疫检测。所开发的均相时间分辨荧光（HTRF）检测方法耗时短（10分钟）且操作简单（无需洗涤步骤），能够检测出浓度为10 ng/mL的F1和2.5 ng/mL的Vag。这些检测方法已成功应用于检测血培养瓶中不同菌株生长过程中产生的疾病生物标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/a98c860015c9/pathogens-10-00285-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/3b29a200a74d/pathogens-10-00285-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/68572830cf81/pathogens-10-00285-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/9707be6fc5da/pathogens-10-00285-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/0e083109b1b0/pathogens-10-00285-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/a98c860015c9/pathogens-10-00285-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/3b29a200a74d/pathogens-10-00285-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/68572830cf81/pathogens-10-00285-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/9707be6fc5da/pathogens-10-00285-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/0e083109b1b0/pathogens-10-00285-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6118/8001648/a98c860015c9/pathogens-10-00285-g005.jpg

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BMC Infect Dis. 2020 Jan 30;20(1):90. doi: 10.1186/s12879-020-4812-7.
2
Improved production of monoclonal antibodies against the LcrV antigen of using FACS-aided hybridoma selection.使用荧光激活细胞分选术辅助的杂交瘤筛选提高抗耶尔森氏菌外膜蛋白V抗原单克隆抗体的产量。
J Biol Methods. 2018 Nov 7;5(4):e100. doi: 10.14440/jbm.2018.257. eCollection 2018.
3
Postexposure Administration of a Yersinia pestis Live Vaccine for Potentiation of Second-Line Antibiotic Treatment Against Pneumonic Plague.
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Pathogens. 2022 Feb 16;11(2):255. doi: 10.3390/pathogens11020255.
鼠疫耶尔森氏菌活疫苗的暴露后给药增强二线抗生素治疗肺鼠疫的效果。
J Infect Dis. 2019 Aug 30;220(7):1147-1151. doi: 10.1093/infdis/jiz260.
4
Novel Phage Display-Derived Anti-Abrin Antibodies Confer Post-Exposure Protection against Abrin Intoxication.新型噬菌体展示衍生抗相思豆毒素抗体可提供相思豆毒素中毒后的保护作用。
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5
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6
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7
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10
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