Yi Eunjue, Sunaguchi Naoki, Lee Jeong Hyeon, Kim Chul-Yong, Lee Sungho, Jheon Sanghoon, Ando Masami, Seok Yangki
Department of Thoracic and Cardiovascular Surgery, Korea University Anam Hospital, Seoul 02841, Korea.
Department of Radiological and Medical Laboratory Sciences, Graduate School of Medicine, Nagoya University, Nagoya 464-8601, Japan.
Diagnostics (Basel). 2021 Mar 10;11(3):487. doi: 10.3390/diagnostics11030487.
The aim of this study was to evaluate the clinical implication of synchrotron radiation imaging techniques for human lung adenocarcinoma in comparison with pathologic examination. A refraction-based tomographic imaging technique called the X-ray dark-field imaging (XDFI) method was used to obtain computed tomographic images of human lung adenocarcinoma at the beam line at Photon Factory BL 14B at the High Energy Accelerator Research Organization (KEK) in Tsukuba, Japan. Images of normal lung tissue were also obtained using the same methods and reconstructed as 3D images. Both reconstructed images were compared with pathologic examinations from histologic slides which were made with identical samples. Pulmonary alveolar structure including terminal bronchioles, alveolar sacs, and vasculatures could be identified in synchrotron radiation images of normal lung. Hyperplasia of interstitial tissue and dysplasia of alveolar structures were noticed in images of lung adenocarcinoma. Both synchrotron radiation images were considerably correlated with images from histologic slides. Lepidic patterns of cancer tissue were distinguished from the invasive area in synchrotron radiation images of lung adenocarcinoma. Refraction-contrast tomographic techniques using synchrotron radiation could provide high-resolution images of lung adenocarcinoma which are compatible with those from pathologic examinations.
本研究的目的是与病理检查相比，评估同步辐射成像技术对人肺腺癌的临床意义。一种基于折射的断层成像技术，称为X射线暗场成像（XDFI）方法，用于在日本筑波高能加速器研究组织（KEK）的光子工厂BL 14B束线处获取人肺腺癌的计算机断层图像。正常肺组织的图像也使用相同方法获取并重建为三维图像。将这两种重建图像与用相同样本制作的组织学切片的病理检查结果进行比较。在正常肺的同步辐射图像中可以识别出包括终末细支气管、肺泡囊和脉管系统在内的肺泡结构。在肺腺癌图像中注意到间质组织增生和肺泡结构发育异常。两种同步辐射图像与组织学切片图像均有显著相关性。在肺腺癌的同步辐射图像中，癌组织的鳞屑样模式与浸润区域得以区分。使用同步辐射的折射对比断层技术可以提供与病理检查结果相符的高分辨率肺腺癌图像。
