比较转录组分析鉴定出与点带石斑鱼黑斑图案形成相关的候选基因()。

Comparative Transcriptome Analysis Identifies Candidate Genes Related to Black-Spotted Pattern Formation in Spotted Scat ().

作者信息

Lin Xiaozhan, Tian Changxu, Huang Yang, Shi Hongjuan, Li Guangli

机构信息

Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China.

Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species, Guangdong Provincial Engineering Laboratory for Mariculture Organism Breeding, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals, Guangdong Ocean University, Zhanjiang 524088, China.

出版信息

Animals (Basel). 2021 Mar 10;11(3):765. doi: 10.3390/ani11030765.

DOI:10.3390/ani11030765

PMID:33802016

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8001731/

Abstract

Spotted scat () is an economically important marine aquaculture and ornamental fish species in Asia, especially in southeast China. In this study, skin transcriptomes of were obtained for three types of skin, including black-spotted skin (A), non-spotted skin (B) and caudal fin (C). A total of nine complementary DNA (cDNA) libraries were obtained by Illumina sequencing. Bioinformatics analysis revealed that 1358, 2086 and 487 genes were differentially expressed between A and B, A and C, and B and C, respectively. The results revealed that there were 134 common significantly differentially expressed genes (DEGs) and several key genes related to pigment synthesis and pigmentation, including , , , , , and . Of these, some DEGs were associated with pigmentation-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as tyrosine metabolism, melanogenesis, the Wnt signaling pathway and the mitogen-activated protein kinase (MAPK) signaling pathway. The results will facilitate understanding the molecular mechanisms of skin pigmentation differentiation in and provide valuable information for skin coloration, especially the formation of spotted patterns on other marine fish species.

摘要

点带石斑鱼（）是亚洲一种具有重要经济价值的海水养殖和观赏鱼类，在中国东南部尤为如此。在本研究中，获取了点带石斑鱼三种类型皮肤的转录组，包括黑斑皮肤（A）、无斑皮肤（B）和尾鳍（C）。通过Illumina测序共获得了9个互补DNA（cDNA）文库。生物信息学分析表明，A与B、A与C、B与C之间分别有1358、2086和487个基因差异表达。结果显示，共有134个共同的显著差异表达基因（DEGs）以及几个与色素合成和色素沉着相关的关键基因，包括、、、、、和。其中，一些DEGs与色素沉着相关的京都基因与基因组百科全书（KEGG）通路有关，如酪氨酸代谢、黑色素生成、Wnt信号通路和丝裂原活化蛋白激酶（MAPK）信号通路。这些结果将有助于理解点带石斑鱼皮肤色素沉着分化的分子机制，并为皮肤颜色，特别是其他海水鱼类斑点图案的形成提供有价值的信息。