Bae Hyocheol, Park Sunwoo, Yang Changwon, Song Gwonhwa, Lim Whasun
Institute of Animal Molecular Biotechnology and Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Korea.
Department of Food and Nutrition, College of Science and Technology, Kookmin University, Seoul 02707, Korea.
Antioxidants (Basel). 2021 Mar 3;10(3):379. doi: 10.3390/antiox10030379.
Phytosterols, which are present in a variety of foods, exhibit various physiological functions and do not have any side effects. Here, we attempted to identify functional role of campesterol in regulation of oxidative stress by leading to cell death of ovarian cancer. We investigated the effects of campesterol on cancer cell aggregation using a three-dimensional (3D) culture of human ovarian cancer cells. The effects of campesterol on apoptosis, protein expression, proliferation, the cell cycle, and the migration of these cells were determined to unravel the underlying mechanism. We also investigated whether campesterol regulates mitochondrial function, the generation of reactive oxygen species (ROS), and calcium concentrations. Our results show that campesterol activates cell death signals and cell death in human ovarian cancer cells. Excessive calcium levels and ROS production were induced by campesterol in the two selected ovarian cancer cell lines. Moreover, campesterol suppressed cell proliferation, cell cycle progression, and cell aggregation in ovarian cancer cells. Campesterol also enhanced the anticancer effects of conventional anticancer agents. The present study shows that campesterol can be used as a novel anticancer drug for human ovarian cancer.
植物甾醇存在于多种食物中，具有多种生理功能且无任何副作用。在此，我们试图通过导致卵巢癌细胞死亡来确定菜油甾醇在氧化应激调节中的功能作用。我们使用人卵巢癌细胞的三维（3D）培养研究了菜油甾醇对癌细胞聚集的影响。测定了菜油甾醇对这些细胞凋亡、蛋白质表达、增殖、细胞周期和迁移的影响，以阐明其潜在机制。我们还研究了菜油甾醇是否调节线粒体功能、活性氧（ROS）的产生和钙浓度。我们的结果表明，菜油甾醇激活人卵巢癌细胞中的细胞死亡信号并导致细胞死亡。在两种选定的卵巢癌细胞系中，菜油甾醇诱导了过量的钙水平和ROS产生。此外，菜油甾醇抑制卵巢癌细胞的细胞增殖、细胞周期进程和细胞聚集。菜油甾醇还增强了传统抗癌药物的抗癌效果。本研究表明，菜油甾醇可作为一种新型的人卵巢癌抗癌药物。
